Dear all, The TOP camera is installed. So simultaneous imaging of (for the moment) GFP/RFP is again possible. If you would like to simultaneously image CFP/YFP the beamsplitter needs to be changed. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Cc: "Daniel Van Damme" <dadam@psb.vib-ugent.be> Sent: Friday, May 24, 2019 11:57:40 AM Subject: Top Camera arrived! UPDATE Spinning disc: Top camera not working
Dear all,
Good news: the top camera of the spinning disc arrived today. It has been sent directly from Hamamatsu Japan to us (without the extra step to go first to Hamamatsu UK).
I will install and check it next week (as the system is occupied the whole day today).
Gr, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Monday, April 1, 2019 5:57:48 PM Subject: [Confocal] UPDATE Spinning disc: Top camera not working
Dear all,
Here an update about the top camera of the spinning disc. Unfortunatelly, still some waiting time ahead. I will be in June at the earliest. Hopefully the Brexit will not cause any extra delay.
This is ongoing or has to come: - repair in Japan is ongoing. This will take 2-3 months (March till May or June) - camera will be send to Hamamatsu UK - Hamamatsu UK will send this camera to us (hoping that we will not have delay by Brexit) (there is no way we can skip this step as Hamamatsu never sends directly to a customer).
I share your frustration over the long time it takes for this repair, which is dued to a combination of different factors: - Different companies are involved: The Hamamatsu camera was purchased via Perkin Elmer UK, so any defect on the camera has to go to Hamamatsu UK. If they can not fix the problem it has to go to Hamamatsu Japan. After repair it has to follow this order again (Japan to UK to Belgium). Each transfer involves quit some steps: each company request first a quote for the sending, which requires an order form. Followed by a quote for checking and again an order form. This restarts when sending to another company is needed. All these steps causes a lot of communication, administration and delays. - Type of camera: As we have a dual camera setup it is crucial to have two identical cameras. As these types of cameras (C9100-13) are not produced anymore, we can not get a new one. Dued to the high cost for repair ( £11,500) we have been looking also for a demo camera, which seemed after testing not good enough.
My apologies for the long waiting time. I will be informed when the camera will be send to Hamamatsu UK, and than I should have a better idea about the exact return time.
Best regards,
Evelien
From: "Evelien Mylle" <evmyl@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Tuesday, July 24, 2018 4:40:39 PM Subject: [Confocal] UPDATE Spinning disc: Top camera not working
Dear all,
The repair of the top camera will take quit some time. After several tests, it points out that it's a camera issue. The camera has been sent to Hamamatsu UK. Depending on the problem, sending the camera to Hamamatsu Japan could be required. As you can imagine, this could take quit some time, probably up to 8-10 weeks.
You still can use the rear camera (what most people use I think), but top and dual camera mode is not possible.
Greetings,
Evelien
From: "Evelien Mylle" <evmyl@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Friday, June 15, 2018 5:38:52 PM Subject: [Confocal] Spinning disc: Top camera not working
Dear spinning disc,
The Top camera is not working. Please keep this in mind for your experiments.
You still can use the rear camera (what most people use I think), but top and dual camera mode is not working. As usual I will keep you informed and hope to have this solved rather fast.
Greetings,
Evelien
Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
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