Dear confocal users,

I find it very disappointing to see that very important instructions are not followed by some users.  One of the criteria for accessing a confocal is to turn the system on and off correctly, and thus be up to date when changes occur.   Determining that this is missing is something that I find very serious.

There has been a very explicit request not to turn off the argon laser. This has been communicated via email and there is also a note on the laser itself.
It has happened twice since then that the laser has been turned off anyway, which meant that the next user had to wait a half to full day before the laser was turned on.   

So I don't know what the cause of the not following is: not being informed (not registered in the confocal mailing list?), ignoring notes on the device,...?
Suggestions to have everyone follow these instructions are always welcome!

Best regards,

Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>, "Ignacio ACHON" <igach@psb.vib-ugent.be>
Sent: Friday, October 30, 2020 4:39:44 PM
Subject: [Confocal] olympus confocal: IMPORTANT!! switching off adapted        procedure!!!
Dear Olympus users,
@ Ignachio, can you confirm as you are the last user today. (I could not find you in the lab)

Please follow the instructions in picture below or in attach in order to switch ONLY the 559 and fluorescence lamp off, so LEAVING THE ARGON LASER ON!! 

Reason is the following: 
The argon laser took this morning more than one hour to get properly working.   
THis, together with the non -working 515 laser, makes me thinks that the argon laser is at his end.  
From experience, not getting the laser on is often one of the first signs.  Once it's on, it's all OK. 
That's why I adjust the switching off procedure by leaving the laser on!  It is a bit risky, with a possible lockdown in prospect, but I don't want to take the risk of not being able to activate the laser on Monday.  The reservations are full for next week, and possibly these are experiments that will be finalized next week.

Do switch off the 559 and the  fluorescence lamp!  
See cadaped switching off procedure below: 


Gr,
Evelien


Gr,

Evelien

Evelien Mylle - Labmanager/ microscopy technician
Advanced Live Cell Imaging

VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 74
www.psb.ugent.be

Genome editing, cutting-edge technology for a sustainable agriculture


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