Dear all, The argon laser of the olympus ignited again, but the output of the 488, 458 and 515nm laser lines is down to about 15%. You therefore will have to use more laser power to image and you will not be able to compare the signal with previous experiments. Please also note that we are currently only keeping the argon laser continuously on, not the diode (405, 559 and 635nm) laser lines, which should be switched off whenever not used. cheers, daniel -- ================================================================== Daniel Van Damme Tel:+32 (0)9 331 39 33 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM dadam@psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================