From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>
Sent: Friday, May 10, 2019 12:07:03 PM
Subject: [Confocal] Zeiss710: 'jumps' in line formation of image

Dear Zeiss 710 confocal users,

There is an issue with the scanning on the Zeiss 710, causing some 'jumps' in the image formation.  Please take a look at the pictures.  The jumps are indicated with an arrow.

I assume this is also related to the noise we recently hear during the scanning.

A temporary solution (or perhaps partially) would be to scan at rotation 90° in stead of rotation 0, as this makes more use of the Y scanhead in stead of the X scanhead, which is probably a bit overworked.

Also the noise in this setup is less.  In the test sample I observed jumps when scanning with rotation 0° , but not with rotation 90°.  (but could be also dued to sample as in this direction jumps are more difficult to see)

The company is contacted and I will update you.

Gr,

Evelien

Evelien Mylle - Labmanager/ microscopy technician
Advanced Live Cell Imaging

VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 74
www.psb.ugent.be

Genome editing, cutting-edge technology for a sustainable agriculture

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