Dear all, Below some information about the use of the SP8: reload settings - bidirectional scanning - saving info. Please read them well, as they can help you. These info will be also available at the right wall of the SP8 and in the folder \\psb.ugent.be\shares\research\images\images_cameras\confocal\Help Leica SP8. 1. Reload settings: It's recommanded NOT to reload settings from images from before 17-01-2020 as this is from an older software version. In some cases this could create conflict with the software. 2. Bidirectional scanning: 3. Save in temporary file In case of problems, please contact Daniel or me. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture