[Confocal] Leica SP2: Argon laser not working anymore

Dear SP2 users, The argon laser (with lines 458,476,488,496,514) is not working anymore. The other lines (561, 594 and 633) are fine. Please keep this in mind for your experiments. The PI imaging, which is often done on this confocal, can still be done using the 561 to excite. GFP-imaging is not possible anymore. I will inform you when the new laser is installed. Best greetings, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

Dear all, The argon laser is working again without any actions. Whether this is 'the start of the end' of the argon laser is something that has to be evaluated over the coming days-weeks. So, please inform Daniel or me in case if you notice something. Thanks in advance, Best regards, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Monday, September 30, 2019 11:01:57 AM Subject: [Confocal] Leica SP2: Argon laser not working anymore
Dear SP2 users,
The argon laser (with lines 458,476,488,496,514) is not working anymore. The other lines (561, 594 and 633) are fine. Please keep this in mind for your experiments. The PI imaging, which is often done on this confocal, can still be done using the 561 to excite. GFP-imaging is not possible anymore.
I will inform you when the new laser is installed.
Best greetings,
Evelien
Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
Genome editing, cutting-edge technology for a sustainable agriculture
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Evelien Mylle