Dear all, The filter at pos 10 of the spinning disc got loose and I had to remove it. The system runs, but currently you cannot use pos10 at all (there is nothing in there now). Also, when using the dual camera, rigorously check the calibration as I had to remove the camera to get to the filter wheel. Have fun imaging, d Daniel Van Damme - Group leader Advanced Live Cell Imaging VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 13 www.psb.ugent.be