Dear SP8 users, If no transmitted light is observed on your sample at microscope, please check position of shutter at the top of microscope (see picture). By mounting your sample on the microscope, this shutter could be accidently pushed up. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture