Dear Leica SP2 users, The 515 laser seems to be unstable: sometimes there is no output at all, whereas this is the case for all other lasers. When this happens, could you move the slider for the % of the 515 laser (in the software) a few times from 0 to 100%. Check if this helps. Please inform me whenever you have this issue. Thanks in advance, Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]