Dear all, If you use the bidirectional scanning , please check and adapt if needed the Phase X value . This value should be on 24.08 and not on 0, which is recently the case. Not having the correct value results in bad images, see an example of the transmitted light below. I will look into getting the correct value again as default value, as it used to be. For the moment this note is also stitched to the wall at the SP8 to remind you about the number. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture