Calendar Name: seminars
Scheduled for: Monday, May 6 2013, 11:00 - 12:30
Event text: Prof Waltraud Schulze
Department of Plant Systems Biology
University of Hohenheim
Stuttgart
GERMANY
Details: �Into the unknown: Dynamic phosphoproteomics in
identifying novel functions for a plant receptor kinase�
ABSTRACT
Growth and development of plants depends on uptake and
assimilation of nutrients. Many assimilation products of
macronutrients are not only essential for plant growth
but also have signaling functions. Similarly to hormones
they can trigger and control various metabolic,
physiological and developmental processes. The nutrient
and sugar induced signal transduction events upstream of
transcription are less well understood. Since signaling
pathways involve protein phosphorylation,
modification-dependent changes in protein abundance and
activity can be important features in signaling
networks. Therefore, the analysis of phosphorylation
events of membrane proteins under different nutrient
regimes, and their dynamics was analyzed with the aim to
identify proteins involved in early nutrient signaling
processes. Using systems biology approaches such as
clustering of time-course data and respective network
reconstruction we are able to identify candidate
proteins as key players in the plant signalling
networks. Based on the network model, we generate new
biological hypotheses on novel protein functions that we
experimentally verified in tailored physiological and
cell biological experiments. An example of a novel
function for a plant receptor kinase in context of
sucrose-induced responses will be discussed.
