- Seminars - maillist.psb.ugent.be

PSB event reminder
by contact＠psb.vib-ugent.be 12 Mar '12

12 Mar '12

Calendar Name: seminars Scheduled for: Monday, March 12 2012, 11:00 - 11:30 Event text: Prof Silke Robatzek The Sainsbury Laboratory Norwich UNITED KINGDOM Details: �Plants communicating with pathogens: membranes in motion and cellular defense� ABSTRACT Cell surface receptors of plant cells constitute recognition sites to detect invading pathogens and to active defenses. Arabidopsis FLS2 encodes the receptor kinase for bacterial flagellin (flg22) and is required for immunity against a broad-spectrum of potentially pathogenic bacteria. Upon flg22 perception FLS2 accumulates at plasma membrane microdomains and is internalized. Although receptor trafficking became a focus of research in the past years, there is largely nothing known about downstream molecules and regulatory components of receptor endocytosis. I will present quantitative high throughput confocal imaging in plants and will discuss results from our current research, which addresses the identity of the FLS2 endosome, molecular components regulating FLS2 endocytosis, and the interception of FLS2 endocytosis and flg22 signaling. These results provide good evidences for a role of late endosomes/multivesicular bodies in plant immunity. To further study cellular defenses we focus on stomatal closure as a first layer of plant immunity. I will describe approaches using high throughput confocal imaging for the genetic dissection of stomata response pathways, and present exciting data how pathogens inhibit stomatal closure by interference with hormonal signaling. Altogether, these studies allow us to better understand the molecular mechanisms underlying the subcellular changes in plant-pathogen interactions.

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PSB event reminder
by contact＠psb.vib-ugent.be 10 Mar '12

10 Mar '12

Calendar Name: seminars Scheduled for: Monday, March 12 2012, 11:00 - 11:30 Event text: Prof Silke Robatzek The Sainsbury Laboratory Norwich UNITED KINGDOM Details: �Plants communicating with pathogens: membranes in motion and cellular defense� ABSTRACT Cell surface receptors of plant cells constitute recognition sites to detect invading pathogens and to active defenses. Arabidopsis FLS2 encodes the receptor kinase for bacterial flagellin (flg22) and is required for immunity against a broad-spectrum of potentially pathogenic bacteria. Upon flg22 perception FLS2 accumulates at plasma membrane microdomains and is internalized. Although receptor trafficking became a focus of research in the past years, there is largely nothing known about downstream molecules and regulatory components of receptor endocytosis. I will present quantitative high throughput confocal imaging in plants and will discuss results from our current research, which addresses the identity of the FLS2 endosome, molecular components regulating FLS2 endocytosis, and the interception of FLS2 endocytosis and flg22 signaling. These results provide good evidences for a role of late endosomes/multivesicular bodies in plant immunity. To further study cellular defenses we focus on stomatal closure as a first layer of plant immunity. I will describe approaches using high throughput confocal imaging for the genetic dissection of stomata response pathways, and present exciting data how pathogens inhibit stomatal closure by interference with hormonal signaling. Altogether, these studies allow us to better understand the molecular mechanisms underlying the subcellular changes in plant-pathogen interactions.

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Seminar Prof Bruno Cammue 22/03
by Delphine Verspeel 09 Mar '12

09 Mar '12

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at <https://maillist.psb.ugent.be/mailman/listinfo/seminars> https://maillist.psb.ugent.be/mailman/listinfo/seminars. If you do not wish to receive this information anymore, please unsubscribe from future mailings at <https://maillist.psb.ugent.be/mailman/options/seminars> https://maillist.psb.ugent.be/mailman/options/seminars. cid:946540515@15122008-0D1C PSB cid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 09 Mar '12

09 Mar '12

Calendar Name: seminars Scheduled for: Friday, March 9 2012, 14:00 - 15:30 Event text: Prof Wout Boerjan VIB, Dept. of Plant Systems Biology Ghent University Gent BELGIUM Details: �Engineering lignin for biofuels� ABSTRACT Lignin is an aromatic polymer that is abundantly present in secondarily-thickened plant cell walls. It confers rigidity to the wall and allows transport of water and solutes through the vascular system. However, lignin plays a negative role in a number of agro-industrial processes, such as pulping and forage digestibility and it is also one of the most important limiting factors in the conversion of plant cell walls to fermentable sugars in the process to liquid bio-fuels. Hence, significant research efforts have been devoted to understand the lignin biosynthesis pathway and the effects of down-regulation of individual steps of this pathway on lignin amount and composition, and on wood properties. These studies have shown that lignin amount and composition can be altered, often without apparent negative consequences for plant health. The first results obtained from poplar field trials will be used to illustrate how lignin engineering can improve biomass processing. Furthermore, we have taken a systems biology approach involving transcriptomics and metabolomics in Arabidopsis to study how the plant, as a biological system, copes with perturbations in the lignin biosynthesis pathway, to reveal regulation within this pathway and to identifying novel genes with potential economic value. Finally, an example will be presented how natural variation in the poplar germplasm can be exploited in a reverse genetics strategy to identify gene function and to accelerate tree genetic improvement.

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PSB event reminder
by contact＠psb.vib-ugent.be 07 Mar '12

07 Mar '12

Calendar Name: seminars Scheduled for: Friday, March 9 2012, 14:00 - 15:30 Event text: Prof Wout Boerjan VIB, Dept. of Plant Systems Biology Ghent University Gent BELGIUM Details: �Engineering lignin for biofuels� ABSTRACT Lignin is an aromatic polymer that is abundantly present in secondarily-thickened plant cell walls. It confers rigidity to the wall and allows transport of water and solutes through the vascular system. However, lignin plays a negative role in a number of agro-industrial processes, such as pulping and forage digestibility and it is also one of the most important limiting factors in the conversion of plant cell walls to fermentable sugars in the process to liquid bio-fuels. Hence, significant research efforts have been devoted to understand the lignin biosynthesis pathway and the effects of down-regulation of individual steps of this pathway on lignin amount and composition, and on wood properties. These studies have shown that lignin amount and composition can be altered, often without apparent negative consequences for plant health. The first results obtained from poplar field trials will be used to illustrate how lignin engineering can improve biomass processing. Furthermore, we have taken a systems biology approach involving transcriptomics and metabolomics in Arabidopsis to study how the plant, as a biological system, copes with perturbations in the lignin biosynthesis pathway, to reveal regulation within this pathway and to identifying novel genes with potential economic value. Finally, an example will be presented how natural variation in the poplar germplasm can be exploited in a reverse genetics strategy to identify gene function and to accelerate tree genetic improvement.

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PSB event reminder
by contact＠psb.vib-ugent.be 02 Mar '12

02 Mar '12

Calendar Name: seminars Scheduled for: Friday, March 2 2012, 14:00 - 15:30 Event text: Prof Takashi Hashimoto Graduate School of Biological Sciences Nara Institute of Science and Technology Takayama-cho Ikoma JAPAN Details: �Organization of cortical microtubule arrays in Arabidopsis� ABSTRACT Microtubules are nucleated from dispersed cortical regions in interphase plant cells, where the majority of nucleation events occur from the -tubulin-containing sites on the pre-existing microtubules as branching patterns. The minus-ends of newly formed daughter microtubules are usually released from sites of nucleation by the action of the microtubule severing complex katanin, and the free microtubules are then transported on the cortex by polymer treadmilling. Subsequent microtubule-microtubule interactions promote microtubule bundling and ordering, which establishes particular patterns of interphase cortical arrays. With special interests in helical microtubule arrays, we have been studying possible mechanisms and particular molecules that underlie organization of cortical microtubule arrays. M. Nakamura, D. Ehrhardt, and T. Hashimoto (2010) Microtubule and katanin dependent dynamics of microtubule nucleation complexes in the Arabidopsis cortical array. Nature Cell Biol. 12: 1064-1070. M. Nakamura, and T. Hashimoto (2009) A mutation in the Arabidopsis -tubulin-containing complex subunit causes helical growth and abnormal microtubule branching. J. Cell Sci. 122: 2208-2217. T. Ishida, Y. Kaneko, M. Iwano, and T. Hashimoto (2007) Helical microtubule arrays in a collection of twisting tubulin mutants of Arabiodpsis thaliana. Proc. Natl. Acad. Sci. USA 104: 8544-8549.

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PSB event reminder
by contact＠psb.vib-ugent.be 02 Mar '12

02 Mar '12

Calendar Name: seminars Scheduled for: Friday, March 2 2012, 14:00 - 15:30 Event text: Prof Taku Demura Graduate School of Biological Sciences Nara Institute of Science and Technology Takayama-cho Ikoma JAPAN RIKEN Biomass Engineering Program Suehiro-cho Tsurumi-ku Yokohama JAPAN Details: �Transcriptional regulatory network for woody cellulosic biomass/secondary cell wall production� ABSTRACT Woody cellulosic biomass derived from plant secondary cell walls is one of the most widely utilized natural materials on our planet. Recent researches found several key regulatory genes controlling xylem cell differentiation, such as NAC (VND7, NST1, SND1, etc) and myb (MYB46, MYB83, etc) transcription factors, which comprise a fine transcriptional regulatory network. Based on the finding, we have established several experimental systems for analyzing secondary cell wall formation: Arabidopsis plants and BY-2 cells expressing the VND7 fused to a glucocorticoid-receptor inducibly produce secondary cell walls. I would like to discuss about the possibility to modify quantity and quality of woody biomass using such the basic knowledge. Ohtani et al. (2011) A NAC domain protein family contributing to the regulation of wood formation in poplar. Plant J 67: 499-512. Yamaguchi et al. (2010) VASCULAR-RELATED NAC-DOMAIN6 and VNSCULAR-RELATED NAC-DOMAIN7 effectively induce transdifferentiation into xylem vessel elements under control of an induction system. Plant Physiol 153: 915-924. M. Kubo et al. (2005) Transcription switches for protoxylem and metaxylem vessel formation. Genes Dev. 19: 1855-1860.

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PSB event reminder
by contact＠psb.vib-ugent.be 29 Feb '12

29 Feb '12

Calendar Name: seminars Scheduled for: Friday, March 2 2012, 14:00 - 15:30 Event text: Prof Takashi Hashimoto Graduate School of Biological Sciences Nara Institute of Science and Technology Takayama-cho Ikoma JAPAN Details: �Organization of cortical microtubule arrays in Arabidopsis� ABSTRACT Microtubules are nucleated from dispersed cortical regions in interphase plant cells, where the majority of nucleation events occur from the -tubulin-containing sites on the pre-existing microtubules as branching patterns. The minus-ends of newly formed daughter microtubules are usually released from sites of nucleation by the action of the microtubule severing complex katanin, and the free microtubules are then transported on the cortex by polymer treadmilling. Subsequent microtubule-microtubule interactions promote microtubule bundling and ordering, which establishes particular patterns of interphase cortical arrays. With special interests in helical microtubule arrays, we have been studying possible mechanisms and particular molecules that underlie organization of cortical microtubule arrays. M. Nakamura, D. Ehrhardt, and T. Hashimoto (2010) Microtubule and katanin dependent dynamics of microtubule nucleation complexes in the Arabidopsis cortical array. Nature Cell Biol. 12: 1064-1070. M. Nakamura, and T. Hashimoto (2009) A mutation in the Arabidopsis -tubulin-containing complex subunit causes helical growth and abnormal microtubule branching. J. Cell Sci. 122: 2208-2217. T. Ishida, Y. Kaneko, M. Iwano, and T. Hashimoto (2007) Helical microtubule arrays in a collection of twisting tubulin mutants of Arabiodpsis thaliana. Proc. Natl. Acad. Sci. USA 104: 8544-8549.

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PSB event reminder
by contact＠psb.vib-ugent.be 29 Feb '12

29 Feb '12

Calendar Name: seminars Scheduled for: Friday, March 2 2012, 14:00 - 15:30 Event text: Prof Taku Demura Graduate School of Biological Sciences Nara Institute of Science and Technology Takayama-cho Ikoma JAPAN RIKEN Biomass Engineering Program Suehiro-cho Tsurumi-ku Yokohama JAPAN Details: �Transcriptional regulatory network for woody cellulosic biomass/secondary cell wall production� ABSTRACT Woody cellulosic biomass derived from plant secondary cell walls is one of the most widely utilized natural materials on our planet. Recent researches found several key regulatory genes controlling xylem cell differentiation, such as NAC (VND7, NST1, SND1, etc) and myb (MYB46, MYB83, etc) transcription factors, which comprise a fine transcriptional regulatory network. Based on the finding, we have established several experimental systems for analyzing secondary cell wall formation: Arabidopsis plants and BY-2 cells expressing the VND7 fused to a glucocorticoid-receptor inducibly produce secondary cell walls. I would like to discuss about the possibility to modify quantity and quality of woody biomass using such the basic knowledge. Ohtani et al. (2011) A NAC domain protein family contributing to the regulation of wood formation in poplar. Plant J 67: 499-512. Yamaguchi et al. (2010) VASCULAR-RELATED NAC-DOMAIN6 and VNSCULAR-RELATED NAC-DOMAIN7 effectively induce transdifferentiation into xylem vessel elements under control of an induction system. Plant Physiol 153: 915-924. M. Kubo et al. (2005) Transcription switches for protoxylem and metaxylem vessel formation. Genes Dev. 19: 1855-1860.

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Seminar Prof Silke Robatzek 12/03
by Delphine Verspeel 27 Feb '12

27 Feb '12

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at <https://maillist.psb.ugent.be/mailman/listinfo/seminars> https://maillist.psb.ugent.be/mailman/listinfo/seminars. If you do not wish to receive this information anymore, please unsubscribe from future mailings at <https://maillist.psb.ugent.be/mailman/options/seminars> https://maillist.psb.ugent.be/mailman/options/seminars. cid:946540515@15122008-0D1C PSB cid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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