- Seminars - maillist.psb.ugent.be

PSB event reminder
by contact＠psb.vib-ugent.be 08 Mar '10

08 Mar '10

Calendar Name: seminars Scheduled for: Monday, March 8 2010, 11:00 - 12:30 Event text: Dr Stig Uggerh�j Andersen Department of Molecular Biology University of Aarhus Aarhus C DENMARK Details: "SHOREmap: simultaneous mapping and mutation identification by deep sequencing" ABSTRACT Identification of causative point mutations after forward genetic mutant screens typically begins with genetic mapping, followed by transformation rescue or candidate gene sequencing. We present a one step alternative: performing hundreds of thousands of genotyping assays while sequencing all candidate genes. This is accomplished by deep sequencing of a pool of F2 progeny obtained by crossing to a polymorphic strain and does not require prior knowledge of mapping markers. The application of high-throughput sequencing shortens the overall time required for genetic mapping from months to weeks and, importantly, greatly reduces investigator hands-on time. The steps requiring investigator input are: DNA isolation (1 day), library preparation and validation (4 days), Illumina cluster generation and sequencing (2 days), and data analysis (1 day). Once the mapping population has been established, the present method therefore allows a single investigator to identify a causative mutation within only eight working days � approximately an order of magnitude faster than with conventional methods.

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PSB event reminder
by contact＠psb.vib-ugent.be 05 Mar '10

05 Mar '10

Calendar Name: seminars Scheduled for: Monday, March 8 2010, 11:00 - 12:30 Event text: Dr Stig Uggerh�j Andersen Department of Molecular Biology University of Aarhus Aarhus C DENMARK Details: "SHOREmap: simultaneous mapping and mutation identification by deep sequencing" ABSTRACT Identification of causative point mutations after forward genetic mutant screens typically begins with genetic mapping, followed by transformation rescue or candidate gene sequencing. We present a one step alternative: performing hundreds of thousands of genotyping assays while sequencing all candidate genes. This is accomplished by deep sequencing of a pool of F2 progeny obtained by crossing to a polymorphic strain and does not require prior knowledge of mapping markers. The application of high-throughput sequencing shortens the overall time required for genetic mapping from months to weeks and, importantly, greatly reduces investigator hands-on time. The steps requiring investigator input are: DNA isolation (1 day), library preparation and validation (4 days), Illumina cluster generation and sequencing (2 days), and data analysis (1 day). Once the mapping population has been established, the present method therefore allows a single investigator to identify a causative mutation within only eight working days � approximately an order of magnitude faster than with conventional methods.

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upcoming seminars March
by Nathalie Vanden Hautte 01 Mar '10

01 Mar '10

Dear All Enclosed you can find an overview of our upcoming seminars in March. Kind regards Nathalie ____________________________________________ Nathalie Vanden Hautte Administrative Assistant VIB, Department of Plant Systems Biology Ghent University Technologiepark 927 9052 Gent Belgium Phone: +32/9/33.13.800 Fax: +32/33.13.809 Http://www.psb.ugent.be nahau(a)psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 26 Feb '10

26 Feb '10

Calendar Name: seminars Scheduled for: Friday, February 26 2010, 14:00 - 15:30 Event text: Joachim Jacob St�phane Plaisance BITS VIB Bioinformatics Training and Service Facility Details: "The VIB BITS facility, your guide in bioinformatic analyses" Presentation by Joachim Jacob and St�phane Plaisance Within the fast evolving discipline of biotechnology emerges a broad need for understanding and managing vast amounts of data. This data can be your sequencing data, your microarray data, your mass-spec data. But it involves also efficiently mining literature sources and the numerous online repositories. Let BITS be your guide in this bewildering maze of bioinformatics! The VIB Bioinformatics and Training Service facility assist all VIB scientists in efficient access and use of relevant software. We have licensed several software packages which are available to the whole VIB community. The BITS training service offer several training sessions, aimed at the wet-lab scientist, starting from low-level training sessions, such as �Bioinformatics introduction training� and �Basic Vector NTI training�, to advanced courses, such as �Advanced Microarray data analysis� and several programming language training sessions. Training material will be continuously available at our website, so you can always fall back on our online expertise. In addition, you can contact BITS data mining service for custom data mining of your high-throughput data. During this short seminar, we will present the training courses scheme for 2010. We will also give a short demonstration of plant-oriented data mining cases. And most importantly, at the end of the seminar, you will have the opportunity to suggest us new training sessions and to let us know with which data you struggle. As such, BITS can meet your future training and data mining needs! An overview of our services and software offered by BITS can be obtained through our website, www.bits.vib.be. Check out our training poll! Hope to see you soon, on our training sessions or online! The BITS team

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PSB event reminder
by contact＠psb.vib-ugent.be 24 Feb '10

24 Feb '10

Calendar Name: seminars Scheduled for: Friday, February 26 2010, 14:00 - 15:30 Event text: Joachim Jacob St�phane Plaisance BITS VIB Bioinformatics Training and Service Facility Details: "The VIB BITS facility, your guide in bioinformatic analyses" Presentation by Joachim Jacob and St�phane Plaisance Within the fast evolving discipline of biotechnology emerges a broad need for understanding and managing vast amounts of data. This data can be your sequencing data, your microarray data, your mass-spec data. But it involves also efficiently mining literature sources and the numerous online repositories. Let BITS be your guide in this bewildering maze of bioinformatics! The VIB Bioinformatics and Training Service facility assist all VIB scientists in efficient access and use of relevant software. We have licensed several software packages which are available to the whole VIB community. The BITS training service offer several training sessions, aimed at the wet-lab scientist, starting from low-level training sessions, such as �Bioinformatics introduction training� and �Basic Vector NTI training�, to advanced courses, such as �Advanced Microarray data analysis� and several programming language training sessions. Training material will be continuously available at our website, so you can always fall back on our online expertise. In addition, you can contact BITS data mining service for custom data mining of your high-throughput data. During this short seminar, we will present the training courses scheme for 2010. We will also give a short demonstration of plant-oriented data mining cases. And most importantly, at the end of the seminar, you will have the opportunity to suggest us new training sessions and to let us know with which data you struggle. As such, BITS can meet your future training and data mining needs! An overview of our services and software offered by BITS can be obtained through our website, www.bits.vib.be. Check out our training poll! Hope to see you soon, on our training sessions or online! The BITS team

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seminar Prof Wen-Hui Shen 15/03
by Nathalie Vanden Hautte 17 Feb '10

17 Feb '10

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars <https://maillist.psb.ugent.be/mailman/listinfo/seminars> . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars <https://maillist.psb.ugent.be/mailman/options/seminars> . VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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seminar Dr Stig U. Andersen 08/03
by Nathalie Vanden Hautte 04 Feb '10

04 Feb '10

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars <https://maillist.psb.ugent.be/mailman/listinfo/seminars> . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars <https://maillist.psb.ugent.be/mailman/options/seminars> . VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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seminar Joachim Jacob & Stéphane Plaisance 26/02
by Nathalie Vanden Hautte 01 Feb '10

01 Feb '10

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars <https://maillist.psb.ugent.be/mailman/listinfo/seminars> . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars <https://maillist.psb.ugent.be/mailman/options/seminars> . VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 28 Jan '10

28 Jan '10

Calendar Name: seminars Scheduled for: Thursday, January 28 2010, 11:00 - 12:30 Event text: Dr Lars �stergaard John Innes Centre Norwich UK Details: "Cell fate specification guided by hormonal and genetic interplay during fruit development" ABSTRACT Mobile signals provided by hormones and morphogens are essential to organise multicellular structures. Despite detailed knowledge on the dynamics of specific hormones in specific cellular environments, not much is known about the interactions between hormonal and genetic events that lead to proper organ formation. The Arabidopsis fruit provides an outstanding model system to study organ patterning and cell differentiation with dramatically different cell types located immediately next to each other. Work on Arabidopsis fruit development has been impressively successful in identifying key genetic regulators; however, the data clearly indicate that in order to get an integrated view of fruit patterning, it is necessary to understand the role of hormones in the process. We use Arabidopsis and Brassica fruits as models to study how genetic and hormonal activities interact to form a complex organ. Results will be presented on the importance of both local hormone biosynthesis and distribution, on communication between tissues to coordinate growth and how these processes are controlled. A high-definition map of hormone distribution during fruit development is currently under construction. This map will be used to construct a mathematical model of fruit development, which is likely to provide exciting new insight into how hormones control organ growth and development.

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PSB event reminder
by contact＠psb.vib-ugent.be 26 Jan '10

26 Jan '10

Calendar Name: seminars Scheduled for: Thursday, January 28 2010, 11:00 - 12:30 Event text: Dr Lars �stergaard John Innes Centre Norwich UK Details: "Cell fate specification guided by hormonal and genetic interplay during fruit development" ABSTRACT Mobile signals provided by hormones and morphogens are essential to organise multicellular structures. Despite detailed knowledge on the dynamics of specific hormones in specific cellular environments, not much is known about the interactions between hormonal and genetic events that lead to proper organ formation. The Arabidopsis fruit provides an outstanding model system to study organ patterning and cell differentiation with dramatically different cell types located immediately next to each other. Work on Arabidopsis fruit development has been impressively successful in identifying key genetic regulators; however, the data clearly indicate that in order to get an integrated view of fruit patterning, it is necessary to understand the role of hormones in the process. We use Arabidopsis and Brassica fruits as models to study how genetic and hormonal activities interact to form a complex organ. Results will be presented on the importance of both local hormone biosynthesis and distribution, on communication between tissues to coordinate growth and how these processes are controlled. A high-definition map of hormone distribution during fruit development is currently under construction. This map will be used to construct a mathematical model of fruit development, which is likely to provide exciting new insight into how hormones control organ growth and development.

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