''Regulation of tenuazonic acid production in a plant pathogenic fungus, Pyricularia oryzae''

Prof Hiroyuki Osada

Department of Pharmaceutical Sciences

Thursday, July 14, 2022
15:00

Rice blast disease is the most important disease of rice worldwide because the disease results in total crop failure. The disease is caused by Pyricularia oryzae (Magnaporthe oryzae), a pathogenic fungus. P. oryzae has many secondary metabolism genes but the

most secondary metabolites are scarcely produced or not produced under laboratory conditions. Secondary metabolite production might be strictly regulated to produce under specific environmental conditions, such as upon the infection.

In my presentation, the induced production of tenuazonic acid, a mycotoxin, in P. oryzae upon the infection will be discussed.

1) Identification of tenuazonic acid biosynthetic gene cluster in P. oryzae.

2) Biosynthetic machinery for tenuazonic acid synthesis.

3) Battle between pathogenic fungus and rice plant.

References

1    Yun, C. S., Motoyama, T. & Osada, H. Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme. Nature Commun 6, 8758 (2015).

2    Yun, C. S., Motoyama, T. & Osada, H. Regulatory Mechanism of Mycotoxin Tenuazonic Acid Production in Pyricularia oryzae. ACS Chem Biol 12, 2270-2274 (2017).

3    Yun, C. S. et al. Unique features of the ketosynthase domain in a nonribosomal peptide synthetase-polyketide synthase hybrid enzyme, tenuazonic acid synthetase 1. J Biol Chem 295, 11602-11612 (2020).

4    Kashiwa, T., Motoyama, T., Yoshida, K., Yun, C. S. & Osada, H. Tenuazonic acid production is dispensable for virulence, but its biosynthetic gene expression pattern is associated with the infection of Pyricularia oryzae. Biosci Biotechnol Biochem 86, 135-139 (2021).

5    Motoyama, T., Yun, C. S. & Osada, H. Biosynthesis and biological function of secondary metabolites of the rice blast fungus Pyricularia oryzae. J Ind Microbiol Biotechnol 48, kuab058 (2021).

Jozef Schell seminar room
Technologiepark 71 - 9052 

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