''Plant microRNAs: biogenesis and roles in growth and development''

Prof Javier Palatnik

Institute of Molecular and Cellular Biology of Rosario (IBR)

ARGENTINA

Monday, September 26, 2022
11:00

MicroRNAs are essential endogenous regulators of gene expression in plants and animals. They are generated from foldback precursors by the microprocessor, whose main component is DICER-LIKE1. The mature miRNAs of ~21 nt length are incorporated into ARGONAUTE complexes, which they then guide to target mRNAs that are subsequently cleaved or translationally arrested. In contrast to their stereotypical animal counterparts, plant microRNA precursors are very heterogeneous in size and shape. We have discovered that plant microRNAs can be generated by different processing modes which depend on precise sequence and structural features in the precursors of these small RNAs.

Many evolutionary conserved plant microRNAs regulate transcription factors involved in plant development. MicroRNA miR396 regulates GROWTH-REGUALTING FACTORS (GRFs), which are plant-specific transcription factors. The microRNA miR396-GRF module has been implicated in the promotion of organ growth and crop yield. We have been studying the integration of the miR396 regulatory network in different plant developmental programs. Interestingly, while miR396 regulates cell proliferation in arabidopsis leaves, in the root meristem it aids to control and maintain the stem cell population. A general view of the microRNA biogenesis pathway, as well as the roles of the miR396 regulatory network will be presented.

Jozef Schell seminar room
Technologiepark 71 - 9052 

Genome editing, cutting-edge technology for a sustainable agriculture