Calendar Name:	seminars
Scheduled for:	Tuesday, March 26 2013, 14:00 - 15:30
Event text:	Dr Dani?le Werck Institute of Plant Molecular Biology CNRS UPR 2357 Strasbourg FRANCE
Details:	?Versatility and fast evolution of the cytochrome P450 enzymes in the metabolism of monoterpenols? ABSTRACT The sequencing of plant genomes has revealed that cytochromes P450 (CYPs) form the largest family of plant enzymes in secondary metabolism and that most of their functions were still unknown. This pointed to a very poor understanding of the plant metabolism. We therefore designed complementary strategies to track the overlooked aspects of the secondary metabolism in the model plant Arabidospsis thaliana. One of them is a predictive map of P450 functions in plant metabolism, based on the analysis of gene (co)-expression in publicly available Affymetrix ATH1 microarray data (www-ibmp.u-strasbg.fr/~CYPedia/). Based on CYPedia predictions, a group of co-expressed terpene synthases (TPSs) and CYPs were predicted to be involved in common floral terpenoid metabolism. All four genes were found mainly expressed upon anthesis in the stamen and petal. Investigation of the encodedenzymes in recombinant systems (yeast and Nicotiana benthamiana) and analysis of null and overexpressor mutants revealed new functions of TPS and P450 enzymes in the metabolism of linalool and derived compounds. This work was extended to a more systematic exploration of the family of CYP76 enzymes in Arabidopsis and Catharanthus roseus (in the latter case in connexion with the metabolism of secoirido?ds), which revealed functional versatility, redundancy, specialisation and fast evolution of monoterpenol oxydases, as well as their role in herbicide metabolism.