[Seminars] PSB event reminder
contact at psb.vib-ugent.be
contact at psb.vib-ugent.be
Thu May 23 09:10:01 CEST 2013
Calendar Name: seminars
Scheduled for: Thursday, May 23 2013, 11:00 - 12:30
Event text: Prof Ann Depicker
VIB, Dept. of Plant Systems Biology
Ghent University
Gent
BELGIUM
Details: Plant transgenes: integration, silencing and
overexpression
ABSTRACT
Plant transgene expression group
I will describe the general picture of plant
transgenesis by presenting the latest results obtained
in the group on the integration and expression of
transgenes in plants. Although Agrobacterium mediated
transformation is widely used, the system is certainly
not completely understood and there are still many
unanswered questions.
A first big unknown is how and when the variable number
of T-DNA copies are integrated at random positions in
the plant chromosomes. However, using the new
recombination technologies, the target position and copy
number of transgenes can now better be controlled.
A second enigma is how a particular transgene may show
up to 1000 fold different expression levels in
independently obtained transformants. It became clear
that the transgene expression level is not only
controlled by its sequence such as the quality of the
promotor and transcript maturation signals but also by
the transgene insertion site, the copy number, genetic
background and several expression controlling
mechanisms. Studying transgenes, it was found that
chromatin modifications, DNA scanning mechanisms and
posttranscriptional control all modulate the expression
level. Particular determinants are inverted repeats and
thresholds for aberrantly processed transcripts in
nucleus and cytoplasm.. We will present 2 examples of
how various epigenetic mechanisms can change transgene
expression levels.
I will conclude by illustrating the application of plant
transgene expression for molecular farming of valuable
recombinant proteins. For this, we will focus on the
protective ability of IgA antibodies when provided in
the feed against enterotoxigenic Escherichia coli
infection in weaned piglets.
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