April 2018 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] SP8X free till 13:00
by Lucas ALVES NEUBUS CLAUS 11 Apr '18

11 Apr '18

Lucas Alves Neubus Claus - Predoctoral fellow Brassinosteriods VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Spinning disk free from 13:30 - 17:30
by Joanna Winkler 09 Apr '18

09 Apr '18

Greetigns, Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica SP2: choose 'company' profile in stead of personal profile when launching the software
by Evelien Mylle 06 Apr '18

06 Apr '18

Dear Leica SP2 users, Please adapt the procedure for starting up the Leica SP2 software: choose 'company' profile in stead of 'personal' profile. (see attach for printscreen). When you choose personal profile, what used to be the standard way, you get an error not allowing to startup the software. It's very strange that this could have happened, it seems that settings were messed up. I assume/ hope that this is not causing any conflict with company settings, but this is the only way how the system can work for the moment. I contacted the company to ask whether we can leave it like that, or if it should be adapted. Best regards, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Spinning disk free from now on
by Joanna Winkler 06 Apr '18

06 Apr '18

Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica SP8: bidirectional scanning requires phase correction
by Evelien Mylle 06 Apr '18

06 Apr '18

Dear Leica SP8 users, Since the upgrade of the software you need to adapt the phaseX correction when doing the bidirectional scanning (see attach how to do). Before the upgrade this was (mostly) never needed, but now this is the case. Without adaptation you get a very strange image (there is a shift from the startposition for each line compared to the previous line, it seems a bit like a splitted image). (when you see it you will notice that it is not good!) There is no fixed value to where you need to change, but probably it is in the range of 20. Look at the image till it looks nice. I will contact the company to ask if we can go back to the situation where no adaptation is required. This information is also available on following folder: [ file://psb.ugent.be/shares/research/images/images_cameras/confocal/Problems_Leica%20SP8/MAIL%20EVMYL | \\psb.ugent.be\shares\research\images\images_cameras\confocal\Problems_Leica SP8\MAIL EVMYL ] In case of problems, don't hesitate to contact Daniel or me. Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] SP8X free till 11:00
by Roman HUDECEK 06 Apr '18

06 Apr '18

happy imaging Roman Hudecek - Predoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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