- confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] Leica SP8 cancelled today
by Joanna Winkler 29 May '18

29 May '18

Today 16:00-18:30, my plants are too small Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

2 1

[Confocal] SD free tomorrow afternoon, feel free to use
by Evelien Mylle 28 May '18

28 May '18

Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] Zeiss 710: the pathway is not longer blocked, but difference focus at microscope and confocal scanning
by Evelien Mylle 28 May '18

28 May '18

Dear 710 users, The problem of blocked pathway is (partially) solved: - Pathway is no longer blocked. - You will still get 2 errors, that you can ignore (one about tube lens and one about 'not expected answer'). You can ignore these errors. - The only thing you have to take into account is: focussing through the microscope will NOT give you a focused image when scanning. Keep in mind that you will have to adapt the focus a lot when switching to scanning mode. (sometimes a lot especially when imaging with the 60x lens) This is because the engine of the tube lens at the bottom of the microscope - required for keeping the same focus when switching between microscope view and scanning view - is not working properly anymore. Therefore we didn't had the appropriate tube lens and we got the error messages. This also caused the pathway to be blocked. As temporary solution the tube lens has been fixed to the scanning mode. The error messages stays because at start up the software always checks the tube lens (which he can not find anymore for the moment). Also the focus will not be adapted when switching between microscope view and scanning view. The engine is ordered and will normally be installed also during maintenance at the end of the month. In case of problems, don't hesitate to contact Daniel or me. Greetings, Evelien > From: "Daniel Van Damme" <dadam(a)psb.vib-ugent.be> > To: "research" <research(a)psb.vib-ugent.be> > Cc: "Evelien Mylle" <evmyl(a)psb.vib-ugent.be> > Sent: Friday, April 27, 2018 1:55:01 PM > Subject: Zeiss 710 down > Dear all, > The Zeiss710 confocal is down. > There is something blocking the light path. > Zeiss has been notified and we will let you know when it is fixed. > cheers, > d > Prof. Dr. ir. Daniel Van Damme - Group leader > Advanced Live Cell Imaging > VIB-UGent Center for Plant Systems Biology > Ghent University > Technologiepark 927 - 9052 Ghent - Belgium > Tel. +32(0)9 331 39 13 > [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 1

[Confocal] 710 free until 12:00
by Alvaro Fernandez 28 May '18

28 May '18

Feel free to take it. Alvaro Alvaro Daniel Fernandez Fernandez - Predoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] 710 is free until 17:00 Sunday
by ke xu 26 May '18

26 May '18

Signal are too weak to work with. Free to take. Ke Xu - Predoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)93313999 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] Leica SP8 free from 14h30 till end of the day, also NO timelaps ON
by Anaxi Houbaert 25 May '18

25 May '18

Seedlings aren't at the right stage Anaxi Houbaert - Predoctoral fellow Brassinosteroids VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] Leica SP8 tomorrow 9-13 cancelled
by Joanna Winkler 17 May '18

17 May '18

feel free to take it, Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

Re: [Confocal] Zeiss 710: What to do in case if argon laser doesn't work
by Evelien Mylle 17 May '18

17 May '18

> Dear all, > To follow up the problem we had with the argon laser we need to find out what > exactly is not working from the argon laser. > Therefore it is important to have the following information (if the argon laser > is not working): > - which colour do you see at the box of the argon laser: purple with a bit of > orange or only orange? > - which LEDs are on at the front panel > See attach for more info. > Please contact Daniel or me in case of problems. If we are not around, you could > take a picture of the box of the argon laser (to see the colour) and of the > front panel. > Gr, > Evelien >> From: "Evelien Mylle" <evmyl(a)psb.ugent.be> >> To: Confocal(a)psb.ugent.be >> Cc: "Daniel Van Damme" <dadam(a)psb.vib-ugent.be> >> Sent: Wednesday, May 16, 2018 10:05:36 AM >> Subject: For the moment argon laser is working: Re:Zeiss 710: argon laser not >> working >> Hello, >> Today the argon laser is working again. Perhaps there is an unstable component >> or ...? >> We have to follow this up. So please keep Daniel and me informed in case if you >> encounter again a problem. >> Thanks, >> Evelien >>> From: "Evelien Mylle" <evmyl(a)psb.ugent.be> >>> To: Confocal(a)psb.ugent.be >>> Cc: "Daniel Van Damme" <dadam(a)psb.vib-ugent.be> >>> Sent: Tuesday, May 15, 2018 6:12:02 PM >>> Subject: Zeiss 710: argon laser not working >>> Dear confocal users, >>> The argon laser of the Zeiss 710 was not working this afternoon. >>> No idea about the reason, so don't hesitate to try again. You never know... >>> Greetings, >>> Evelien >>> Evelien Mylle - Lab Manager / Technician >>> Advanced Live Cell Imaging >>> VIB-UGent Center for Plant Systems Biology >>> Ghent University >>> Technologiepark 927 - 9052 Ghent - Belgium >>> Tel. +32(0)9 331 39 34 >>> [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] SP8 free till 11h
by Amna Mhamdi 17 May '18

17 May '18

Hi, feel free to use the SP8, I only need it at 11h. Best regards, Amna Amna M'Hamdi - Postdoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 25 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

2 1

[Confocal] Zeiss 710: argon laser not working
by Evelien Mylle 16 May '18

16 May '18

Dear confocal users, The argon laser of the Zeiss 710 was not working this afternoon. No idea about the reason, so don't hesitate to try again. You never know... Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 1