- confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] SD: slow imaging
by Evelien Mylle 14 May '18

14 May '18

Dear spinning disc users, We have a problem with our spinning disc as the imaging takes much longer than should be the case. F.ex. One channel and one slice makes the imaging around 500 msec longer than what should be the case (here is no need for changing filter or beamsplitter, so only the exposure time determines the imaging time). These observations were done with the rear camera. It's not checked whether also the top camera does this. Please keep this in mind when doing your experiments. In principle everything can still be done, but much slower. Depending on your experiment this can be doable or problematic. Company is informed and I will keep you informed. Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] 710 FREE today from 14.30 to 17.30
by Marta Cubria Radio 14 May '18

14 May '18

Feel free to use it! Cheers, Marta Cubria Radio - Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 14 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] “Luxaflex” usb stick found at SP8
by Jos Wendrich 08 May '18

08 May '18

Jos Wendrich, PhD Post-Doc PSB, VIB-UGent Vascular Development Technologiepark Zwijnaarde 927 9052 Ghent, Belgium Email: jos.wendrich(a)psb.ugent.be Phone: +32 9 331 39 43 http://www.psb.ugent.be/vascular-development Google Scholar: https://scholar.google.nl/citations?user=Cqs0p58AAAAJ&hl=nl Research Gate: https://www.researchgate.net/profile/Jos_Wendrich LinkedIn: https://nl.linkedin.com/in/jos-wendrich-29642416

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[Confocal] Leica SP8 free today from 9:00 - 12:30
by Astrid Gadeyne 08 May '18

08 May '18

Astrid Gadeyne - Postdoctoral fellow Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 74 www.psb.ugent.be

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[Confocal] spinning disk free
by Joanna Winkler 07 May '18

07 May '18

>From now on, Greetigns, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Zeiss 710: argon laser doesn't not always switch on - if on, do NOT switch off!
by Evelien Mylle 04 May '18

04 May '18

Dear confocal users, The argon laser of Zeiss 710 has problems to switch on. As the laser has almost 7000 working hours, I think this is the beginning of the end for this laser. Monday there was a similar problem, but on Tuesday, when the technician of Zeiss was here, the switching on was fine. Today again there was a problem with switching on. The first sign of degradation is often problem with switching on. I can only advice to try several times to get it on, either by restarting up completely. Or, and I think this could be more more helpfull, by switching the system on, leave it on and hoping that the laser will go on after a while. If someone succeeds in getting the laser on, please DO NOT SWITCH OFF!!! (even for the weekend!) It can be left on till replacement will be done. The company will be contacted for the installation of a new laser. Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Looking for people with experience with long timelaps using Leica SP8
by Anaxi Houbaert 02 May '18

02 May '18

Hello dear SP8 confocal users I'm looking for someone who tried long (>12h) timelaps on the SP8. I would like to image cotyledon development using 1.5-2days old seedlings. I would like to hear feedback on the protocol and settings of the microscope. Many thanks in advance! Anaxi Anaxi Houbaert - Predoctoral fellow Brassinosteroids VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica sp8 free
by Joanna Winkler 02 May '18

02 May '18

from now until 7pm (Evelien will not use it today) for training Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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Re: [Confocal] LSM710 is free till 16:00.
by Daniel SAVATIN 25 Apr '18

25 Apr '18

I'll take it. Thanks > From: "Pengliang WEI" <pewei(a)psb.vib-ugent.be> > To: "wetlab" <wetlab(a)psb.vib-ugent.be> > Sent: Wednesday, April 25, 2018 1:32:17 PM > Subject: LSM710 is free till 16:00. > Feel free to take it. > Pengliang Wei - Predoctoral fellow > Cell Cycle > VIB-UGent Center for Plant Systems Biology > Ghent University > Technologiepark 927 - 9052 Ghent - Belgium > Tel. +32(0)9 331 39 63 > [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] tidiness of confocal rooms
by Evelien Mylle 24 Apr '18

24 Apr '18

Dear all, I would like to request you to keep the confocal rooms clean as they are now . All material you take in, should be taken out. The only things that are now on the bench are slides, coverslides, scalpels, gloves and tissues. All other stuff should not stay in the room. All confocal rooms had a proper cleaning and all superfluous stuff has been removed. The benches are now clean and free to prepare your samples. (see ppt in attach). With this fresh clean start, I think it should be realistic to keep it clean. It would be very nice if you would all contribute to this. The rooms will be regularly checked and people will be addressed if they leave stuff in the room. This will be done by the PhD helpers and room responsibles for the corresponding rooms. Thanks in advance, Best regards, Evelien Mylle, Lucas Alves, Gust Bilcke, Amber Lampens, Ambra De Simone Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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