- confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] Leica Sp2: 514 laser is unstable, sometimes it works, sometimes not. Other lasers are fine.
by Evelien Mylle 22 Mar '18

22 Mar '18

Dear confocal lasers, The 514 laser from the Leica SP2 gives unstable output. Sometimes working nicely, other times not. The other lasers are working nicely. Depending on your dye or fluorophore it could be possible to excite your sample by using another laserline. Leica SP2 has also a 496 laser which is the closest to the 514 laser. The company is informed and I will keep you updated. Best regards, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica SP8: panel showing laserlines & selecting laserlines is changed
by Evelien Mylle 22 Mar '18

22 Mar '18

Dear Leica SP8 users, The layout where you select your laserlines is changed dued to upgrade of the LasX software. The way to use is still the same, but it just looks a bit different. In attach more info. Enjoy, Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Exciter free today after 17h until tomorrow 15h
by Rafael Buono 21 Mar '18

21 Mar '18

Best, Rafael Rafael Andrade Buono - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] SP8X free today from 12-13h
by Rafael Buono 21 Mar '18

21 Mar '18

Grab it if you want it. Best, Rafael Rafael Andrade Buono - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] SP8X free from 9:00 - 12:00
by Roman HUDECEK 21 Mar '18

21 Mar '18

happy confocaling :-D Roman Hudecek - Predoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] confocal 710 free from 17:00 to 19:00 today(Tuesday)
by ke xu 20 Mar '18

20 Mar '18

Free to take. Ke Xu - Predoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)93313999 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Exciter: check position of condensor before starting up
by Evelien Mylle 19 Mar '18

19 Mar '18

Dear confocal users, Please check the position of the condensor before use! It should be completely up. You can adapt this by turning the condensor wheel (see attach for picture). If the condensor is too low, there will not be enough space for lowering the stage. The microscope will produce a strange noise as it keeps on trying to lower the stage. At the end this could break the stage!! The condensor is lowered when peope use covered chamber glass for long time imaging (in stead of slide). If things are not clear, please don't hesitate to contact Daniel or me. Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Exciter: fluorescence lamp is changed
by Evelien Mylle 19 Mar '18

19 Mar '18

Dear confocal users, The fluorescence lamp of the Exciter has been replaced by the X-Cite from the Axioimager. (See attach for picture.) You can use the same procedure for switching on as with the previous lamp (button on). You have an extra option to choose the percentage of output of light by turning the black wheel. If you know that the next user will use it within 5 hours, than it's better to leave it on. (with the previous lamp this was within 30 minutes). But if you don't know, make sure that the lamp is not on for the whole night!!! In casy of any problems, don't hesitate to contact Daniel or me. Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] SP8 is free the rest of the afternoon.
by Brecht Wybouw 19 Mar '18

19 Mar '18

Feel free to take it. Otherwise I will turn of the laser. Brecht Wybouw - Predoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 63 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] zeiss LSM 710 free 12-13.30h
by Sarah Langendries 16 Mar '18

16 Mar '18

Feel free to take it Sarah Langendries - Predoctoral fellow Rhizosphere VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 08 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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