Dear all, The 580 beamsplitter is now installed on the spinning disc allowing to simultaneously image GFP/RFP . If you would like to simultaneously image CFP/YFP the beamsplitter needs to be changed to the 509 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). This procedure is described on the last page of the manual ( S:\research\groups\group_alice\CONFOCAL\Info\Manuals ConfocalsPSB\Spinning Disc\Spinning Disc_Manual_V20200828). If not clear, don't hesitate to ask for help. Best regards, Evelien From: "Evelien Mylle" <Evelien.Mylle@psb.ugent.be> To: Confocal@psb.ugent.be Cc: "Daniel Van Damme" <dadam@psb.vib-ugent.be> Sent: Friday, June 14, 2019 4:49:30 PM Subject: SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP BQ_BEGIN Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). This procedure is described on the last page of the manual described S:\research\groups\group_alice\CONFOCAL\Info\Manuals ConfocalsPSB\Spinning Disc\Spinning Disc_Manual_V20200828 If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture BQ_END