Dear all, The Leica SP8 gives sometimes problems when looking through the microscope to your sample: there is less light and you don't get your sample nicely in focus. It can be solved by clicking (several times) on live view (in the LAS X software), and/or by switching the lens, and/or switching between DIC and POL light (microscope setting). Closing and restarting the PC sometimes helps, but not always, and it is very time consuming. So, I would go for the above options. I assume that there is some kind of filter that gets blocked. Keep this in mind when doing your imaging especially if you are doing treatments, as it could take a bit more time to set up your imaging. I will keep you updated. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture