Dear spinning disc users, The shutter for transmitted light doesn't work. But luckily we are able to have the light coming to our sample, meaning that normal imaging still can be done. But make sure when you switch to confocal mode to have the transmitted light off by using the external box (whereas before you could do this by using the shutter). If you forget this, than all the transmitted light will go on to your detector, which will be dramatic for the camera!! In case of questions, don't hesitate to contact Daniel or me. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture