[Confocal] Zeiss710: 'jumps' in line formation of image
Dear Zeiss 710 confocal users, There is an issue with the scanning on the Zeiss 710, causing some 'jumps' in the image formation. Please take a look at the pictures. The jumps are indicated with an arrow. I assume this is also related to the noise we recently hear during the scanning. A temporary solution (or perhaps partially) would be to scan at rotation 90° in stead of rotation 0, as this makes more use of the Y scanhead in stead of the X scanhead, which is probably a bit overworked. Also the noise in this setup is less. In the test sample I observed jumps when scanning with rotation 0° , but not with rotation 90°. (but could be also dued to sample as in this direction jumps are more difficult to see) The company is contacted and I will update you. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture
Dear all, Technician of Zeiss will come on 16/05. Sarah, Tom, Inge, Xiaopeng, Please keep this in mind for your scheduled experiments that day. Johannes will come somewhere that day, but no idea when. Gr, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Friday, May 10, 2019 12:07:03 PM Subject: [Confocal] Zeiss710: 'jumps' in line formation of image
Dear Zeiss 710 confocal users,
There is an issue with the scanning on the Zeiss 710, causing some 'jumps' in the image formation. Please take a look at the pictures. The jumps are indicated with an arrow.
I assume this is also related to the noise we recently hear during the scanning.
A temporary solution (or perhaps partially) would be to scan at rotation 90° in stead of rotation 0, as this makes more use of the Y scanhead in stead of the X scanhead, which is probably a bit overworked. Also the noise in this setup is less. In the test sample I observed jumps when scanning with rotation 0° , but not with rotation 90°. (but could be also dued to sample as in this direction jumps are more difficult to see)
The company is contacted and I will update you.
Gr, Evelien
Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
Genome editing, cutting-edge technology for a sustainable agriculture
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Dear all, The scanners of the Zeiss 710 are replaced and everything is working well again. Thanks Alvaro for informing me about the problem. Enjoy imaging! Gr, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Cc: "Sarah Langendries" <sarah.langendries@psb.vib-ugent.be>, "Xiaopeng LUO" <xiluo@psb.vib-ugent.be>, "Inge De Clercq" <incle@psb.vib-ugent.be> Sent: Friday, May 10, 2019 2:46:24 PM Subject: [Confocal] Visit on 16/05, Re: Zeiss710: 'jumps' in line formation of image
Dear all,
Technician of Zeiss will come on 16/05.
Sarah, Tom, Inge, Xiaopeng, Please keep this in mind for your scheduled experiments that day. Johannes will come somewhere that day, but no idea when.
Gr, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Friday, May 10, 2019 12:07:03 PM Subject: [Confocal] Zeiss710: 'jumps' in line formation of image
Dear Zeiss 710 confocal users,
There is an issue with the scanning on the Zeiss 710, causing some 'jumps' in the image formation. Please take a look at the pictures. The jumps are indicated with an arrow.
I assume this is also related to the noise we recently hear during the scanning.
A temporary solution (or perhaps partially) would be to scan at rotation 90° in stead of rotation 0, as this makes more use of the Y scanhead in stead of the X scanhead, which is probably a bit overworked. Also the noise in this setup is less. In the test sample I observed jumps when scanning with rotation 0° , but not with rotation 90°. (but could be also dued to sample as in this direction jumps are more difficult to see)
The company is contacted and I will update you.
Gr, Evelien
Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
Genome editing, cutting-edge technology for a sustainable agriculture
_______________________________________________ Confocal mailing list Confocal@psb.ugent.be https://maillist.psb.ugent.be/mailman/listinfo/confocal
_______________________________________________ Confocal mailing list Confocal@psb.ugent.be https://maillist.psb.ugent.be/mailman/listinfo/confocal
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Evelien Mylle