[Confocal] Zeiss 710: detector (channel) 2 NOT working
Dear confocal users, Channel or detector 2 from Zeiss 710 is not working. Depending on the number of fluorophores, you can continu imaging but with a small adaptation of the Light path: For imaging up to 2 colours just use the other 2 channels (Ch1 and Ch3). For imaging 3 colours FRAME sequential scanning will be required ( one of the channels will capture 2 colours, or you can even put all colours on one channel). LINE sequential imaging or simultanous imaging will not be possibe. Also note that in case of Smart setup or reuse, you will have to adapt the Light path. Don't hesitate to contact Daniel or me if you would have problems for setting up. Gr, Evelien Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
Hello, Johannes, technician from Zeiss, will come on Tuesday for the exchange of the detector 2 and checking the problem of the unsharp image. Hopefully somewhere in the afternoon both problems will be solved. Gr, Evelien
From: "Evelien Mylle" <evmyl@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Thursday, June 28, 2018 5:06:19 PM Subject: [Confocal] Zeiss 710: detector (channel) 2 NOT working
Dear confocal users,
Channel or detector 2 from Zeiss 710 is not working. Depending on the number of fluorophores, you can continu imaging but with a small adaptation of the Light path: For imaging up to 2 colours just use the other 2 channels (Ch1 and Ch3). For imaging 3 colours FRAME sequential scanning will be required ( one of the channels will capture 2 colours, or you can even put all colours on one channel). LINE sequential imaging or simultanous imaging will not be possibe.
Also note that in case of Smart setup or reuse, you will have to adapt the Light path.
Don't hesitate to contact Daniel or me if you would have problems for setting up.
Gr, Evelien
Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
_______________________________________________ Confocal mailing list Confocal@psb.ugent.be https://maillist.psb.ugent.be/mailman/listinfo/confocal
Dear confocal users, A new detector has been installed and everything is again working as normal. Enjoy imaging! Gr, Evelien
From: "Evelien Mylle" <evmyl@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Thursday, June 28, 2018 5:06:19 PM Subject: [Confocal] Zeiss 710: detector (channel) 2 NOT working
Dear confocal users,
Channel or detector 2 from Zeiss 710 is not working. Depending on the number of fluorophores, you can continu imaging but with a small adaptation of the Light path: For imaging up to 2 colours just use the other 2 channels (Ch1 and Ch3). For imaging 3 colours FRAME sequential scanning will be required ( one of the channels will capture 2 colours, or you can even put all colours on one channel). LINE sequential imaging or simultanous imaging will not be possibe.
Also note that in case of Smart setup or reuse, you will have to adapt the Light path.
Don't hesitate to contact Daniel or me if you would have problems for setting up.
Gr, Evelien
Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
_______________________________________________ Confocal mailing list Confocal@psb.ugent.be https://maillist.psb.ugent.be/mailman/listinfo/confocal
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Evelien Mylle