Hey,Please verify that the proper laser is selected for that detector (where you have the gating on,off and the borders of the gating you also can choose a laser.If still not ok, restart the software. If problem persists,  than I will take a look next week.Please keep me informed. GrEvelien   -------- Oorspronkelijk bericht --------Van: Alvaro Fernandez <Alvaro.Fernandez@psb.vib-ugent.be> Datum: 31/12/20 12:45 (GMT+01:00) Aan: confocal <confocal@psb.vib-ugent.be> Onderwerp: [Confocal] SP8 gating Dear users,Today in the SP8 there was a problem with the gating, there is no difference between gating and no gating so I think the configuration has changed.If there is anyone knowing how to solve it please let us know.Otherwise please consider this when imaging.Cheers,Alvaro Alvaro Daniel Fernandez Fernandez - Predoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 www.psb.ugent.be Genome editing, cutting-edge technology for a sustainable agriculture