Dear all, Here more details on how to fix the problem of not getting your transmitted light to the oculars: Enjoy imaging! Kind regards, Evelien

From: "Daniel Van Damme" <Daniel.VanDamme@psb.ugent.be> To: "research" <research@psb.vib-ugent.be> Cc: "Evelien Mylle" <evelien.mylle@psb.vib-ugent.be> Sent: Friday, June 11, 2021 1:48:54 PM Subject: Re: leica SP8 problems with ocular viewing Dear all,

There is a way to fix the problem on the SP8X that I mentioned before.

In the objectives tab on the screen of the microscope, there is a button that allows switching between eye and scanner. So if the system does not do it on its own, you can do this by clicking that button. We will look into why the system fails to switch, but for now, this is a solution to the problem.

cheers,

d

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From: "Daniel Van Damme" <Daniel.VanDamme@psb.ugent.be> To: "research" <research@psb.vib-ugent.be> Cc: "evelien mylle" <evelien.mylle@psb.vib-ugent.be> Sent: Friday, June 11, 2021 11:08:39 AM Subject: leica SP8 problems with ocular viewing Hi all,

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It is currently not possible to see your sample through the oculars of the Leica SP8. Scanning and imaging works, there is transmitted light, but it simply does not reach the eyepieces.

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Evelien will notify the company and we will keep you updated.

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Sorry for the inconvenience,

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d

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Prof. Dr. ir. Daniel Van Damme - Group leader Advanced Live Cell Imaging

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VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 70 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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