- Seminars - maillist.psb.ugent.be

Seminar Dr Delphine Fleury on Thursday, June 27 at 11 am
by Delphine Verspeel 17 Jun '13

17 Jun '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 13 Jun '13

13 Jun '13

Calendar Name: seminars Scheduled for: Thursday, June 13 2013, 11:00 - 12:30 Event text: Prof Philip Gilmartin Plant Molecular Genetics Faculty of Science University of East Anglia Norwich Norfolk UNITED KINGDOM Details: �Floral heteromorphy in Primula: genetics, genomics and gene identification� ABSTRACT J Li1, 2 . M. Webster1, 2, M. Smith1, 2, J. Cocker1, 2, O. Kent1, 2, J. Wright3, D. Swarbreck3, M. Caccamo3, P. M. Gilmartin1, 2 University of East Anglia 1, John Innes Centre 2 and The Genome Analysis Centre 3, Norwich Research Park, Norwich, UK. Floral heteromorphy is an out-breeding mechanism that is characteristic of, although not limited to, the Primulaceae. The phenomenon was described in detail by Charles Darwin, but first documented over a century earlier. In Primula species, individuals develop one of two forms of flower, known as pin and thrum. Pin flowers are characterised by a long style that presents the stigmatic surface at the mouth of the flower, and contain anthers attached half way down the inner wall of the corolla tube. Thrum flowers have a short style. half the height of that found in pin flowers, and anthers attached to the inner corolla wall at the mouth of the flower. This reciprocal positioning of male and female reproductive structures promotes insect-mediated cross pollination. These developmental characteristics, and other features of floral heteromorphy, including differential pollens size, as well as a sporophytic self-incompatibility system, are controlled by a co-adapted linkage group known as the S locus. Pin plants are homozygous recessive s/s; thrum pants are heterozygous S/s. With the objective of identifying the genes at the S locus that control style length, anther height and pollen size, we have taken a multifaceted approach involving classical genetics, molecular genetics, transcriptomics and genomics. We have identified and characterised a number of genes and polymorphic markers, as well as developmental mutations that are linked to the S locus. Using these sequences and S locus-linked mutant phenotypes we have developed a classical genetic linkage map in Primula vulgaris, spanning the Primula S locus and delineated by flanking markers. Subsequent construction and screening of BAC libraries has enabled us to generate a BAC contig spanning the S locus and to identify pin and thrum specific BACs. Transcriptomic analysis of pin and thrum plants has identified morph specific floral transcripts, and transcriptomic and genomic analyses of linked mutations, including Oakleaf, sepaloid and Hose in Hose has been used to probe the molecular basis of these phenotypes. In addition to these analyses we have undertaken de novo genome sequencing of Primula vulgaris to generate a reference genome and have used genome sequencing of individual parents and pools of pin and thrum progeny to define polymorphic markers surrounding the S locus. Comparative genomic analyses are underway based on de novo sequencing of the four additional UK native species; P. veris, P. elatior, P. farinosa and P. scotica. We have, in parallel, undertaken a large scale of plants derived from fast neutron bombarded P. veris seed to identify long and short homostyle as well as self compatible mutants. We will present our most recent work arising from these studies to highlight progress towards identifying the genes that underpin Darwin�s descriptions of this breeding system.

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PSB event reminder
by contact＠psb.vib-ugent.be 11 Jun '13

11 Jun '13

Calendar Name: seminars Scheduled for: Thursday, June 13 2013, 11:00 - 12:30 Event text: Prof Philip Gilmartin Plant Molecular Genetics Faculty of Science University of East Anglia Norwich Norfolk UNITED KINGDOM Details: �Floral heteromorphy in Primula: genetics, genomics and gene identification� ABSTRACT J Li1, 2 . M. Webster1, 2, M. Smith1, 2, J. Cocker1, 2, O. Kent1, 2, J. Wright3, D. Swarbreck3, M. Caccamo3, P. M. Gilmartin1, 2 University of East Anglia 1, John Innes Centre 2 and The Genome Analysis Centre 3, Norwich Research Park, Norwich, UK. Floral heteromorphy is an out-breeding mechanism that is characteristic of, although not limited to, the Primulaceae. The phenomenon was described in detail by Charles Darwin, but first documented over a century earlier. In Primula species, individuals develop one of two forms of flower, known as pin and thrum. Pin flowers are characterised by a long style that presents the stigmatic surface at the mouth of the flower, and contain anthers attached half way down the inner wall of the corolla tube. Thrum flowers have a short style. half the height of that found in pin flowers, and anthers attached to the inner corolla wall at the mouth of the flower. This reciprocal positioning of male and female reproductive structures promotes insect-mediated cross pollination. These developmental characteristics, and other features of floral heteromorphy, including differential pollens size, as well as a sporophytic self-incompatibility system, are controlled by a co-adapted linkage group known as the S locus. Pin plants are homozygous recessive s/s; thrum pants are heterozygous S/s. With the objective of identifying the genes at the S locus that control style length, anther height and pollen size, we have taken a multifaceted approach involving classical genetics, molecular genetics, transcriptomics and genomics. We have identified and characterised a number of genes and polymorphic markers, as well as developmental mutations that are linked to the S locus. Using these sequences and S locus-linked mutant phenotypes we have developed a classical genetic linkage map in Primula vulgaris, spanning the Primula S locus and delineated by flanking markers. Subsequent construction and screening of BAC libraries has enabled us to generate a BAC contig spanning the S locus and to identify pin and thrum specific BACs. Transcriptomic analysis of pin and thrum plants has identified morph specific floral transcripts, and transcriptomic and genomic analyses of linked mutations, including Oakleaf, sepaloid and Hose in Hose has been used to probe the molecular basis of these phenotypes. In addition to these analyses we have undertaken de novo genome sequencing of Primula vulgaris to generate a reference genome and have used genome sequencing of individual parents and pools of pin and thrum progeny to define polymorphic markers surrounding the S locus. Comparative genomic analyses are underway based on de novo sequencing of the four additional UK native species; P. veris, P. elatior, P. farinosa and P. scotica. We have, in parallel, undertaken a large scale of plants derived from fast neutron bombarded P. veris seed to identify long and short homostyle as well as self compatible mutants. We will present our most recent work arising from these studies to highlight progress towards identifying the genes that underpin Darwin�s descriptions of this breeding system.

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PSB event reminder
by contact＠psb.vib-ugent.be 06 Jun '13

06 Jun '13

Calendar Name: seminars Scheduled for: Thursday, June 6 2013, 11:00 - 12:30 Event text: Dr Markus Teige Department of Molecular Systems Biology (MOSYS) Vienna University Vienna AUSTRIA Details: "Multiple phosphorylations by multiple kinases at multiple locations- how to keep track on cellular signalling in plants?" ABSTRACT Protein phosphorylation is one key regulatory principle in all living organisms: about 30% of all cellular proteins are - at least occasionally - phosphorylated and sequenced genomes encode about 500 to 1000 protein kinases. Moreover, some proteins can be phosphorylated by different kinases at multiple sites, which makes it even more challenging to keep an overview on these regulatory processes. One approach, which helps to get an overview on these diverse processes is to ask where and when these phosphorylation events occur. Many of them will be triggered by specific signals and also take place only in distinct subcellular localizations. Therefore I will start with the discussion of protein kinases, which are involved in the (salt-) stress response at different membrane systems, but which are also active in the cytosol and the nucleus. At all places, there is cross-talk with other signalling pathways and different pathways merge at targets, which are phosphorylated by different kinases. I will present a bZIP transcription factor as one example to illustrate how this could serve as integration point of different signalling pathways, which are used in response to different stresses.

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Seminar Prof Philip Gilmartin on Thursday, June 13 at 11 am
by Delphine Verspeel 05 Jun '13

05 Jun '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 04 Jun '13

04 Jun '13

Calendar Name: seminars Scheduled for: Thursday, June 6 2013, 11:00 - 12:30 Event text: Dr Markus Teige Department of Molecular Systems Biology (MOSYS) Vienna University Vienna AUSTRIA Details: "Multiple phosphorylations by multiple kinases at multiple locations- how to keep track on cellular signalling in plants?" ABSTRACT Protein phosphorylation is one key regulatory principle in all living organisms: about 30% of all cellular proteins are - at least occasionally - phosphorylated and sequenced genomes encode about 500 to 1000 protein kinases. Moreover, some proteins can be phosphorylated by different kinases at multiple sites, which makes it even more challenging to keep an overview on these regulatory processes. One approach, which helps to get an overview on these diverse processes is to ask where and when these phosphorylation events occur. Many of them will be triggered by specific signals and also take place only in distinct subcellular localizations. Therefore I will start with the discussion of protein kinases, which are involved in the (salt-) stress response at different membrane systems, but which are also active in the cytosol and the nucleus. At all places, there is cross-talk with other signalling pathways and different pathways merge at targets, which are phosphorylated by different kinases. I will present a bZIP transcription factor as one example to illustrate how this could serve as integration point of different signalling pathways, which are used in response to different stresses.

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Seminar Dr Markus Teige on Thursday, June 6 at 11 am
by Delphine Verspeel 29 May '13

29 May '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 28 May '13

28 May '13

Calendar Name: seminars Scheduled for: Tuesday, May 28 2013, 11:00 - 12:30 Event text: Prof Ueli Grossniklaus Plant Developmental Genetics Institute of Plant Biology University of Z�rich Z�rich Switzerland Details: �Molecular control of fertilization and interspecific hybridization� ABSTRACT Lena M�ller, Heike Lindner, Sharon A. Kessler, Michael T. Raissig, Hiroko Shimosato-Asano, Ueli Grossniklaus Research in our laboratory focuses on the developmental genetics of plant reproduction, with an emphasis on cellular interactions during double fertilization. I will focus on the reception of the pollen tube by the synergid cells, where the pollen tube arrests growth and ruptures to release the sperm cells. We have isolated and characterized female gametophytic mutants that disrupt pollen tube reception. Pollen tubes that encounter such mutant female gametophytes are unable to rupture and release the sperm cells (Huck et al., Development 130:2149; Kessler et al., Science 330:968). These phenotypes suggest that the female gametophyte controls the behaviour of the male gametophyte (pollen) in this process. One of the mutants, feronia, was shown to affect a receptor-like kinase (Escobar-Restrepo et al., Science 317:656), while another, nortia, disrupts a seven-transmembrane-domain-protein similar to the powdery mildew resistance protein Mlo (Kessler et al., Science 330:968). The identification of additional components in this signal transduction cascade suggest the involvement of glycosylation in this recognition process. Furthermore, interspecific crosses between Brassicaceae can result in a similar phenotype, suggesting the cell-cell interactions during pollen tube reception may be involved in interspecific crossing barriers. Using genome-wide association studies, we have been able to identify a factor that plays a specific role in interspecific compatibility while intraspecific crosses are not affected. Thus, pollen tube reception may be involved in establishing crossing barriers essential to maintain species boundaries similar to sperm-egg interactions in animals.

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Subcribe in mailing list of seminars
by Sara El-Esawe 27 May '13

27 May '13

Quoting seminars-request(a)psb.vib-ugent.be: > Send Seminars mailing list submissions to > seminars(a)psb.ugent.be > > To subscribe or unsubscribe via the World Wide Web, visit > https://maillist.psb.ugent.be/mailman/listinfo/seminars > or, via email, send a message with subject or body 'help' to > seminars-request(a)psb.ugent.be > > You can reach the person managing the list at > seminars-owner(a)psb.ugent.be > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Seminars digest..." > > > Today's Topics: > > 1. PSB event reminder (contact(a)psb.vib-ugent.be) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 26 May 2013 11:10:02 +0200 (CEST) > From: contact(a)psb.vib-ugent.be > To: meetings(a)psb.vib-ugent.be > Subject: [Seminars] PSB event reminder > Message-ID: <20130526091002.1CC8D803B(a)psbweb03.psb.ugent.be> > Content-Type: text/plain; charset="us-ascii" > > Calendar Name: seminars > Scheduled for: Tuesday, May 28 2013, 11:00 - 12:30 > Event text: Prof Ueli Grossniklaus > > Plant Developmental Genetics > Institute of Plant Biology > University of Zürich > > Zürich > Switzerland > Details: ?Molecular control of fertilization and interspecific > hybridization? > > ABSTRACT > Lena Müller, Heike Lindner, Sharon A. Kessler, Michael > T. Raissig, Hiroko Shimosato-Asano, Ueli Grossniklaus > > Research in our laboratory focuses on the developmental > genetics of plant reproduction, with an emphasis on > cellular interactions during double fertilization. I > will focus on the reception of the pollen tube by the > synergid cells, where the pollen tube arrests growth and > ruptures to release the sperm cells. We have isolated > and characterized female gametophytic mutants that > disrupt pollen tube reception. Pollen tubes that > encounter such mutant female gametophytes are unable to > rupture and release the sperm cells (Huck et al., > Development 130:2149; Kessler et al., Science 330:968). > These phenotypes suggest that the female gametophyte > controls the behaviour of the male gametophyte (pollen) > in this process. One of the mutants, feronia, was shown > to affect a receptor-like kinase (Escobar-Restrepo et > al., Science 317:656), while another, nortia, disrupts a > seven-transmembrane-domain-protein similar to the > powdery mildew resistance protein Mlo (Kessler et al., > Science 330:968). The identification of additional > components in this signal transduction cascade suggest > the involvement of glycosylation in this recognition > process. Furthermore, interspecific crosses between > Brassicaceae can result in a similar phenotype, > suggesting the cell-cell interactions during pollen tube > reception may be involved in interspecific crossing > barriers. Using genome-wide association studies, we have > been able to identify a factor that plays a specific > role in interspecific compatibility while intraspecific > crosses are not affected. Thus, pollen tube reception > may be involved in establishing crossing barriers > essential to maintain species boundaries similar to > sperm-egg interactions in animals. > >

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(no subject)
by Sara El-Esawe 27 May '13

27 May '13

Dear Mam/Sir, I would like to subcribe in mailing list. Thanks, Sara

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