- Seminars - maillist.psb.ugent.be

PSB event reminder
by contact＠psb.vib-ugent.be 28 Feb '13

28 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 28 2013, 11:00 - 12:30 Event text: Dr Wolfgang Busch Gregor Mendel Institute Vienna AUSTRIA Details: �Genome-wide association study at the cellular level reveals novel regulators of root development� ABSTRACT Development is ultimately regulated at the cellular level. The coordinated growth and development of cells underlie both form and function of tissues and eventually the whole organism. Assuming that assessing natural variation at the cell type level might facilitate the identification of novel developmental regulators using quantitative genetics, we set out to quantify the cellular architecture of the root in more than 200 natural accessions of Arabidopsis thaliana utilizing automated confocal microscopy. We found widespread natural variation of morphology at the cell-type level and used this variation to conduct genome wide association mapping. Using expression data to identify candidate genes in proximity of significantly associated genome regions, and subsequent characterization of T-DNA insertion lines for those genes, we identified two novel regulators of root development. While the F-Box family gene KUK is involved in coordinating meristem length and terminal cell size, another novel gene, which we designated AUT, encodes a small protein that regulates the width of the root vasculature. Our work not only uncovered two novel players in root development but also demonstrated the feasibility of GWA at the cellular level to identify causal genes for basic biological processes such as development.

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PSB event reminder
by contact＠psb.vib-ugent.be 26 Feb '13

26 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 28 2013, 11:00 - 12:30 Event text: Dr Wolfgang Busch Gregor Mendel Institute Vienna AUSTRIA Details: �Genome-wide association study at the cellular level reveals novel regulators of root development� ABSTRACT Development is ultimately regulated at the cellular level. The coordinated growth and development of cells underlie both form and function of tissues and eventually the whole organism. Assuming that assessing natural variation at the cell type level might facilitate the identification of novel developmental regulators using quantitative genetics, we set out to quantify the cellular architecture of the root in more than 200 natural accessions of Arabidopsis thaliana utilizing automated confocal microscopy. We found widespread natural variation of morphology at the cell-type level and used this variation to conduct genome wide association mapping. Using expression data to identify candidate genes in proximity of significantly associated genome regions, and subsequent characterization of T-DNA insertion lines for those genes, we identified two novel regulators of root development. While the F-Box family gene KUK is involved in coordinating meristem length and terminal cell size, another novel gene, which we designated AUT, encodes a small protein that regulates the width of the root vasculature. Our work not only uncovered two novel players in root development but also demonstrated the feasibility of GWA at the cellular level to identify causal genes for basic biological processes such as development.

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PSB event reminder
by contact＠psb.vib-ugent.be 21 Feb '13

21 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 21 2013, 11:00 - 12:30 Event text: Prof Geert De Jaeger VIB, Dept. of Plant Systems Biology Ghent University Gent BELGIUM Details: �Targeted interactomics in plants through protein complex isolation� ABSTRACT TAPtag technology is the most widely applied tool to pick up in situ protein interactions in a proteome wide setting. Our research team has developed a versatile TAP technology platform for protein complex isolation from plants. We isolated complexes for hundreds of proteins and extensively demonstrated the power of our technology for protein discovery, functional analysis of proteins and protein complexes, and the modelling of protein networks. Complexes are purified from Arabidopsis cell suspension cultures or seedlings and we are currently translating the technology towards crop plants to bring complex purification in a developmental context.

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PSB event reminder
by contact＠psb.vib-ugent.be 19 Feb '13

19 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 21 2013, 11:00 - 12:30 Event text: Prof Geert De Jaeger VIB, Dept. of Plant Systems Biology Ghent University Gent BELGIUM Details: �Targeted interactomics in plants through protein complex isolation� ABSTRACT TAPtag technology is the most widely applied tool to pick up in situ protein interactions in a proteome wide setting. Our research team has developed a versatile TAP technology platform for protein complex isolation from plants. We isolated complexes for hundreds of proteins and extensively demonstrated the power of our technology for protein discovery, functional analysis of proteins and protein complexes, and the modelling of protein networks. Complexes are purified from Arabidopsis cell suspension cultures or seedlings and we are currently translating the technology towards crop plants to bring complex purification in a developmental context.

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Seminar Dr Wolfgang Busch on Thursday, February 28 at 11 am
by Delphine Verspeel 18 Feb '13

18 Feb '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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Seminar Prof Geert De Jaeger on Thursday, February 21 at 11 am
by Delphine Verspeel 14 Feb '13

14 Feb '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 14 Feb '13

14 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 14 2013, 11:00 - 12:30 Event text: Prof Charles White G�n�tique, Reproduction et D�veloppement UMR CNRS 6293 - Clermont Universit� - INSERM U1103 Campus des C�zeaux Universit� Blaise Pascal Clermont Ferrand FRANCE Details: �Roles and mechanisms of recombination in meiosis and mitosis� ABSTRACT O. Da Ines, S. Amiard, C. Charbonnel, K. Abe, F. Degroote, H. Serra, I. Mestiri, C. Goubely, E. Allain, M.E. Gallego and Charles I. White Working with Arabidopsis thaliana, we are studying the roles and mechanisms of genetic recombination - indispensable to the maintenance of genome integrity and an essential source of genetic diversity. Recombination acts to repair DNA damage and in particular, DNA double-strand breaks (DSB). In addition to arising by accident (replication, radiation, mutagens ... ), DSB are naturally present at the ends of linear eukaryotic chromosomes (telomeres) and also produced in a programmed manner in order to induce recombination and genetic exchange (meiosis). DSB are efficiently repaired through multiple individual pathways of homologous or non-homologous recombination and although knowledge of recombination mechanisms has considerably advanced, the complex interrelationships and regulation of these pathways are far from being fully understood. A particularly striking example of the danger of inappropriate recombination is seen in the severe karyotypic and genomic instability in the presence of telomere dysfunction. Current knowledge of roles and mechanisms of recombination in plants will be presented through discussion of our recent results on somatic recombination pathways, responses to telomere dysfunction and the differing requirements for proteins of the Rad51 family in pairing of meiotic centromeric regions and chromosome arms. � Da Ines, O., Abe, K., Goubely, C., Gallego, M. E., & White, C. I. (2012). Differing Requirements for RAD51 and DMC1 in Meiotic Pairing of Centromeres and Chromosome Arms in Arabidopsis thaliana. PLoS Genetics, 8(4), e1002636. � Wei Wei, Zhaoqing Ba, Min Gao, Yang Wu, Yanting Ma, Simon Amiard, Charles I. White, Jannie Michaela Rendtlew Danielsen, Yun-Gui Yang and Yijun Qi. (2012). A Role for Small RNAs in DNA Double-Strand Break Repair. Cell 149: 101-112 � Amiard S, Depeiges A, Allain E, White CI, Gallego ME (2011) Arabidopsis ATM and ATR Kinases Prevent Propagation of Genome Damage Caused by Telomere Dysfunction. Plant Cell, 23:4254-65. � C. Charbonnel, E. Allain, M.E. Gallego and C. White. (2011). Kinetic analysis of post-replicative DSB repair pathways in Arabidopsis. DNA Repair, 10:611-619.

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PSB event reminder
by contact＠psb.vib-ugent.be 12 Feb '13

12 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 14 2013, 11:00 - 12:30 Event text: Prof Charles White G�n�tique, Reproduction et D�veloppement UMR CNRS 6293 - Clermont Universit� - INSERM U1103 Campus des C�zeaux Universit� Blaise Pascal Clermont Ferrand FRANCE Details: �Roles and mechanisms of recombination in meiosis and mitosis� ABSTRACT O. Da Ines, S. Amiard, C. Charbonnel, K. Abe, F. Degroote, H. Serra, I. Mestiri, C. Goubely, E. Allain, M.E. Gallego and Charles I. White Working with Arabidopsis thaliana, we are studying the roles and mechanisms of genetic recombination - indispensable to the maintenance of genome integrity and an essential source of genetic diversity. Recombination acts to repair DNA damage and in particular, DNA double-strand breaks (DSB). In addition to arising by accident (replication, radiation, mutagens ... ), DSB are naturally present at the ends of linear eukaryotic chromosomes (telomeres) and also produced in a programmed manner in order to induce recombination and genetic exchange (meiosis). DSB are efficiently repaired through multiple individual pathways of homologous or non-homologous recombination and although knowledge of recombination mechanisms has considerably advanced, the complex interrelationships and regulation of these pathways are far from being fully understood. A particularly striking example of the danger of inappropriate recombination is seen in the severe karyotypic and genomic instability in the presence of telomere dysfunction. Current knowledge of roles and mechanisms of recombination in plants will be presented through discussion of our recent results on somatic recombination pathways, responses to telomere dysfunction and the differing requirements for proteins of the Rad51 family in pairing of meiotic centromeric regions and chromosome arms. � Da Ines, O., Abe, K., Goubely, C., Gallego, M. E., & White, C. I. (2012). Differing Requirements for RAD51 and DMC1 in Meiotic Pairing of Centromeres and Chromosome Arms in Arabidopsis thaliana. PLoS Genetics, 8(4), e1002636. � Wei Wei, Zhaoqing Ba, Min Gao, Yang Wu, Yanting Ma, Simon Amiard, Charles I. White, Jannie Michaela Rendtlew Danielsen, Yun-Gui Yang and Yijun Qi. (2012). A Role for Small RNAs in DNA Double-Strand Break Repair. Cell 149: 101-112 � Amiard S, Depeiges A, Allain E, White CI, Gallego ME (2011) Arabidopsis ATM and ATR Kinases Prevent Propagation of Genome Damage Caused by Telomere Dysfunction. Plant Cell, 23:4254-65. � C. Charbonnel, E. Allain, M.E. Gallego and C. White. (2011). Kinetic analysis of post-replicative DSB repair pathways in Arabidopsis. DNA Repair, 10:611-619.

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Seminar Prof Charles White on Thursday, February 14 at 11 am
by Delphine Verspeel 04 Feb '13

04 Feb '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 04 Feb '13

04 Feb '13

Calendar Name: seminars Scheduled for: Monday, February 4 2013, 15:00 - 16:30 Event text: Dr John Ryals President CEO Metabolon Inc. Durham North Carolina USA Details: �global metabolomic analysis applied to plant systems� ABSTRACT Metabolon Inc. is a diagnostics and services company offering the industry's a leading biochemical profiling platform. Its patented platform provides a global analysis of complex biological samples for the discovery of markers and pathways associated with drug action and disease. Metabolon has developed the technology to quickly identify and measure all of the biochemicals in a biological sample through its proprietary global processing method. Through the generation and interpretation of data, this method provides a precise understanding of biochemical changes which may explain key biological changes in plant systems. (2011) A sister group contrast using untargeted global metabolomic analysis delineates the biochemical regulation underlying desiccation tolerance in Sporobolus stapfianus. The Plant cell 23, 1231-1248. (2012) The chloroplast triggers developmental reprogramming when MUTS HOMOLOG1 is suppressed in plants. Plant Physiology (2012). "Proteomics and Metabolomics of Arabidopsis Responses to Perturbation of Glucosinolate Biosynthesis." Molecular Plant.

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