- Seminars - maillist.psb.ugent.be

PSB event reminder
by contact＠psb.vib-ugent.be 04 May '13

04 May '13

Calendar Name: seminars Scheduled for: Monday, May 6 2013, 11:00 - 12:30 Event text: Prof Waltraud Schulze Department of Plant Systems Biology University of Hohenheim Stuttgart GERMANY Details: �Into the unknown: Dynamic phosphoproteomics in identifying novel functions for a plant receptor kinase� ABSTRACT Growth and development of plants depends on uptake and assimilation of nutrients. Many assimilation products of macronutrients are not only essential for plant growth but also have signaling functions. Similarly to hormones they can trigger and control various metabolic, physiological and developmental processes. The nutrient and sugar induced signal transduction events upstream of transcription are less well understood. Since signaling pathways involve protein phosphorylation, modification-dependent changes in protein abundance and activity can be important features in signaling networks. Therefore, the analysis of phosphorylation events of membrane proteins under different nutrient regimes, and their dynamics was analyzed with the aim to identify proteins involved in early nutrient signaling processes. Using systems biology approaches such as clustering of time-course data and respective network reconstruction we are able to identify candidate proteins as key players in the plant signalling networks. Based on the network model, we generate new biological hypotheses on novel protein functions that we experimentally verified in tailored physiological and cell biological experiments. An example of a novel function for a plant receptor kinase in context of sucrose-induced responses will be discussed.

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Seminar Prof Waltraud Schulze on Monday, May 6 at 11 am
by Delphine Verspeel 26 Apr '13

26 Apr '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 18 Apr '13

18 Apr '13

Calendar Name: seminars Scheduled for: Thursday, April 18 2013, 11:00 - 12:30 Event text: Prof Dirk Inz� Scientific Director Head of Department VIB, Dept. of Plant Systems Biology Ghent University Gent BELGIUM Details: �LEAF growth: from images to molecular networks� ABSTRACT Growth of plants and plant organs is orchestrated by complex molecular networks that integrates both intrinsic development signals encoded by the genome as well as a wide variety of environmental cues such as light, availability of water and minerals, temperature, e.a. Understanding the molecular composition and topology of these networks ultimately will accelerate advanced breeding and gene engineering for higher yielding crops. We have chosen leaves as a model organ to understand growth and size control mechanisms. As leaf growth is a quantitative trait, several (semi-) automated growth analysis platforms were developed to analyze leaf growth over time. Detailed cellular and molecular analysis of numerous Arabidopsis mutants revealed the existence of at least five mechanisms that contribute to final leaf size: i) the initial size of the leaf primordium; ii) cell cycle duration; iii) the developmental timing of the transition from cell division to cell expansion; iv) the timing of meristemoid division; and v) cell expansion. For each mechanism, multiple genes have been identified that when overexpressed or mutated enhance leaf organ size. For example, cell cycle duration appears to be controlled by the ANAPHASE PROMOTING COMPLEX, a multi-protein E3 ligase that is involved in mitosis. On the other hand the transition from cell division to cell expansion during leaf development is mediated by the gibberellic acid (GA) dependent activity of a chromatin remodeling complex. GA levels in the growth zones are regulated by the activity of two stress responsive transcription factors ERF5 and ERF6 and our experimental data show that these transcription factor have a pivotal role in regulation growth in response to the environment. Furthermore, GA also was shown to have an important function in mediating leaf growth in maize and by engineering GA metabolism maize leaves that have a 40% increase in length were obtained. We will discussed our current understanding of growth regulatory networks and how we can use this information to improve crop yield.

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PSB event reminder
by contact＠psb.vib-ugent.be 16 Apr '13

16 Apr '13

Calendar Name: seminars Scheduled for: Thursday, April 18 2013, 11:00 - 12:30 Event text: Prof Dirk Inz� Scientific Director Head of Department VIB, Dept. of Plant Systems Biology Ghent University Gent BELGIUM Details: �LEAF growth: from images to molecular networks� ABSTRACT Growth of plants and plant organs is orchestrated by complex molecular networks that integrates both intrinsic development signals encoded by the genome as well as a wide variety of environmental cues such as light, availability of water and minerals, temperature, e.a. Understanding the molecular composition and topology of these networks ultimately will accelerate advanced breeding and gene engineering for higher yielding crops. We have chosen leaves as a model organ to understand growth and size control mechanisms. As leaf growth is a quantitative trait, several (semi-) automated growth analysis platforms were developed to analyze leaf growth over time. Detailed cellular and molecular analysis of numerous Arabidopsis mutants revealed the existence of at least five mechanisms that contribute to final leaf size: i) the initial size of the leaf primordium; ii) cell cycle duration; iii) the developmental timing of the transition from cell division to cell expansion; iv) the timing of meristemoid division; and v) cell expansion. For each mechanism, multiple genes have been identified that when overexpressed or mutated enhance leaf organ size. For example, cell cycle duration appears to be controlled by the ANAPHASE PROMOTING COMPLEX, a multi-protein E3 ligase that is involved in mitosis. On the other hand the transition from cell division to cell expansion during leaf development is mediated by the gibberellic acid (GA) dependent activity of a chromatin remodeling complex. GA levels in the growth zones are regulated by the activity of two stress responsive transcription factors ERF5 and ERF6 and our experimental data show that these transcription factor have a pivotal role in regulation growth in response to the environment. Furthermore, GA also was shown to have an important function in mediating leaf growth in maize and by engineering GA metabolism maize leaves that have a 40% increase in length were obtained. We will discussed our current understanding of growth regulatory networks and how we can use this information to improve crop yield.

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Seminar Prof Dirk Inzé on Thursday, April 18 at 11 am
by Delphine Verspeel 03 Apr '13

03 Apr '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 02 Apr '13

02 Apr '13

Calendar Name: seminars Scheduled for: Tuesday, April 2 2013, 14:00 - 15:30 Event text: Dr Peter Bozhkov Department of Plant Biology and Forest Genetics Uppsala BioCenter Swedish University of Agricultural Sciences and Linnean Center for Plant Biology Uppsala SWEDEN Details: �Autophagy: a common mediator of cell death and organismal longevity in plants� ABSTRACT Animals have evolved sophisticated machinery to remove apoptotic bodies and clean up necrotic debris for damping inflammatory response. Plants appear more pragmatic, as they utilize dying cells and cell corpses to construct their bodies, as well as to store and transport nutrients, growth factors, and secondary metabolites. The presence of rigid cell walls and the absence of phagocytosis permit cell corpses to stay post-mortem for a very long time. Disassembly of plant cells during developmental programmed cell death (PCD) is likewise a slow process, which can take up to several days. This mode of PCD is reliant on the gradual engulfment of the cytoplasm by the lytic vacuoles and is hence called vacuolar cell death. We have found that autophagy � a major cellular catabolic process - plays a central role in the execution of vacuolar cell death in the Norway spruce embryo-suspensors. The role of autophagy is cell-autonomous, since down-regulation of ATG genes specifically in the suspensor cells acts as a switch from vacuolar cell death to necrosis, which impairs embryonic patterning and leads to developmental arrest. It has become well known in the last few years that yeasts, worms, flies and mammals with enhanced level of basal autophagy live longer, whereas suppression of autophagy results in premature death or shortened lifespan. Current studies in my laboratory establish autophagy as an important process in the control of plant phenology and longevity. We have found that changes in the expression of some ATG genes can modulate autophagic flux in the leaves of Arabidopsis, which in turn affects phenology and lifespan of the plants. Interestingly, manipulation of both autophagic flux and organismal lifespan can also be achieved in wild type Columbia plants by changing their caloric status through increasing or decreasing light intensity.

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PSB event reminder
by contact＠psb.vib-ugent.be 31 Mar '13

31 Mar '13

Calendar Name: seminars Scheduled for: Tuesday, April 2 2013, 14:00 - 15:30 Event text: Dr Peter Bozhkov Department of Plant Biology and Forest Genetics Uppsala BioCenter Swedish University of Agricultural Sciences and Linnean Center for Plant Biology Uppsala SWEDEN Details: �Autophagy: a common mediator of cell death and organismal longevity in plants� ABSTRACT Animals have evolved sophisticated machinery to remove apoptotic bodies and clean up necrotic debris for damping inflammatory response. Plants appear more pragmatic, as they utilize dying cells and cell corpses to construct their bodies, as well as to store and transport nutrients, growth factors, and secondary metabolites. The presence of rigid cell walls and the absence of phagocytosis permit cell corpses to stay post-mortem for a very long time. Disassembly of plant cells during developmental programmed cell death (PCD) is likewise a slow process, which can take up to several days. This mode of PCD is reliant on the gradual engulfment of the cytoplasm by the lytic vacuoles and is hence called vacuolar cell death. We have found that autophagy � a major cellular catabolic process - plays a central role in the execution of vacuolar cell death in the Norway spruce embryo-suspensors. The role of autophagy is cell-autonomous, since down-regulation of ATG genes specifically in the suspensor cells acts as a switch from vacuolar cell death to necrosis, which impairs embryonic patterning and leads to developmental arrest. It has become well known in the last few years that yeasts, worms, flies and mammals with enhanced level of basal autophagy live longer, whereas suppression of autophagy results in premature death or shortened lifespan. Current studies in my laboratory establish autophagy as an important process in the control of plant phenology and longevity. We have found that changes in the expression of some ATG genes can modulate autophagic flux in the leaves of Arabidopsis, which in turn affects phenology and lifespan of the plants. Interestingly, manipulation of both autophagic flux and organismal lifespan can also be achieved in wild type Columbia plants by changing their caloric status through increasing or decreasing light intensity.

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Seminar Dr Peter Bozhkov on Tuesday, April 2 at 2 pm
by Delphine Verspeel 27 Mar '13

27 Mar '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 26 Mar '13

26 Mar '13

Calendar Name: seminars Scheduled for: Tuesday, March 26 2013, 14:00 - 15:30 Event text: Dr Dani�le Werck Institute of Plant Molecular Biology CNRS UPR 2357 Strasbourg FRANCE Details: �Versatility and fast evolution of the cytochrome P450 enzymes in the metabolism of monoterpenols� ABSTRACT The sequencing of plant genomes has revealed that cytochromes P450 (CYPs) form the largest family of plant enzymes in secondary metabolism and that most of their functions were still unknown. This pointed to a very poor understanding of the plant metabolism. We therefore designed complementary strategies to track the overlooked aspects of the secondary metabolism in the model plant Arabidospsis thaliana. One of them is a predictive map of P450 functions in plant metabolism, based on the analysis of gene (co)-expression in publicly available Affymetrix ATH1 microarray data (http://www-ibmp.u-strasbg.fr/~CYPedia/) Based on CYPedia predictions, a group of co-expressed terpene synthases (TPSs) and CYPs were predicted to be involved in common floral terpenoid metabolism. All four genes were found mainly expressed upon anthesis in the stamen and petal. Investigation of the encodedenzymes in recombinant systems (yeast and Nicotiana benthamiana) and analysis of null and overexpressor mutants revealed new functions of TPS and P450 enzymes in the metabolism of linalool and derived compounds. This work was extended to a more systematic exploration of the family of CYP76 enzymes in Arabidopsis and Catharanthus roseus (in the latter case in connexion with the metabolism of secoirido�ds), which revealed functional versatility, redundancy, specialisation and fast evolution of monoterpenol oxydases, as well as their role in herbicide metabolism.

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PSB event reminder
by contact＠psb.vib-ugent.be 24 Mar '13

24 Mar '13

Calendar Name: seminars Scheduled for: Tuesday, March 26 2013, 14:00 - 15:30 Event text: Dr Dani�le Werck Institute of Plant Molecular Biology CNRS UPR 2357 Strasbourg FRANCE Details: �Versatility and fast evolution of the cytochrome P450 enzymes in the metabolism of monoterpenols� ABSTRACT The sequencing of plant genomes has revealed that cytochromes P450 (CYPs) form the largest family of plant enzymes in secondary metabolism and that most of their functions were still unknown. This pointed to a very poor understanding of the plant metabolism. We therefore designed complementary strategies to track the overlooked aspects of the secondary metabolism in the model plant Arabidospsis thaliana. One of them is a predictive map of P450 functions in plant metabolism, based on the analysis of gene (co)-expression in publicly available Affymetrix ATH1 microarray data (http://www-ibmp.u-strasbg.fr/~CYPedia/) Based on CYPedia predictions, a group of co-expressed terpene synthases (TPSs) and CYPs were predicted to be involved in common floral terpenoid metabolism. All four genes were found mainly expressed upon anthesis in the stamen and petal. Investigation of the encodedenzymes in recombinant systems (yeast and Nicotiana benthamiana) and analysis of null and overexpressor mutants revealed new functions of TPS and P450 enzymes in the metabolism of linalool and derived compounds. This work was extended to a more systematic exploration of the family of CYP76 enzymes in Arabidopsis and Catharanthus roseus (in the latter case in connexion with the metabolism of secoirido�ds), which revealed functional versatility, redundancy, specialisation and fast evolution of monoterpenol oxydases, as well as their role in herbicide metabolism.

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