- Seminars - maillist.psb.ugent.be

PSB event reminder
by contact＠psb.vib-ugent.be 14 Feb '13

14 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 14 2013, 11:00 - 12:30 Event text: Prof Charles White G�n�tique, Reproduction et D�veloppement UMR CNRS 6293 - Clermont Universit� - INSERM U1103 Campus des C�zeaux Universit� Blaise Pascal Clermont Ferrand FRANCE Details: �Roles and mechanisms of recombination in meiosis and mitosis� ABSTRACT O. Da Ines, S. Amiard, C. Charbonnel, K. Abe, F. Degroote, H. Serra, I. Mestiri, C. Goubely, E. Allain, M.E. Gallego and Charles I. White Working with Arabidopsis thaliana, we are studying the roles and mechanisms of genetic recombination - indispensable to the maintenance of genome integrity and an essential source of genetic diversity. Recombination acts to repair DNA damage and in particular, DNA double-strand breaks (DSB). In addition to arising by accident (replication, radiation, mutagens ... ), DSB are naturally present at the ends of linear eukaryotic chromosomes (telomeres) and also produced in a programmed manner in order to induce recombination and genetic exchange (meiosis). DSB are efficiently repaired through multiple individual pathways of homologous or non-homologous recombination and although knowledge of recombination mechanisms has considerably advanced, the complex interrelationships and regulation of these pathways are far from being fully understood. A particularly striking example of the danger of inappropriate recombination is seen in the severe karyotypic and genomic instability in the presence of telomere dysfunction. Current knowledge of roles and mechanisms of recombination in plants will be presented through discussion of our recent results on somatic recombination pathways, responses to telomere dysfunction and the differing requirements for proteins of the Rad51 family in pairing of meiotic centromeric regions and chromosome arms. � Da Ines, O., Abe, K., Goubely, C., Gallego, M. E., & White, C. I. (2012). Differing Requirements for RAD51 and DMC1 in Meiotic Pairing of Centromeres and Chromosome Arms in Arabidopsis thaliana. PLoS Genetics, 8(4), e1002636. � Wei Wei, Zhaoqing Ba, Min Gao, Yang Wu, Yanting Ma, Simon Amiard, Charles I. White, Jannie Michaela Rendtlew Danielsen, Yun-Gui Yang and Yijun Qi. (2012). A Role for Small RNAs in DNA Double-Strand Break Repair. Cell 149: 101-112 � Amiard S, Depeiges A, Allain E, White CI, Gallego ME (2011) Arabidopsis ATM and ATR Kinases Prevent Propagation of Genome Damage Caused by Telomere Dysfunction. Plant Cell, 23:4254-65. � C. Charbonnel, E. Allain, M.E. Gallego and C. White. (2011). Kinetic analysis of post-replicative DSB repair pathways in Arabidopsis. DNA Repair, 10:611-619.

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PSB event reminder
by contact＠psb.vib-ugent.be 12 Feb '13

12 Feb '13

Calendar Name: seminars Scheduled for: Thursday, February 14 2013, 11:00 - 12:30 Event text: Prof Charles White G�n�tique, Reproduction et D�veloppement UMR CNRS 6293 - Clermont Universit� - INSERM U1103 Campus des C�zeaux Universit� Blaise Pascal Clermont Ferrand FRANCE Details: �Roles and mechanisms of recombination in meiosis and mitosis� ABSTRACT O. Da Ines, S. Amiard, C. Charbonnel, K. Abe, F. Degroote, H. Serra, I. Mestiri, C. Goubely, E. Allain, M.E. Gallego and Charles I. White Working with Arabidopsis thaliana, we are studying the roles and mechanisms of genetic recombination - indispensable to the maintenance of genome integrity and an essential source of genetic diversity. Recombination acts to repair DNA damage and in particular, DNA double-strand breaks (DSB). In addition to arising by accident (replication, radiation, mutagens ... ), DSB are naturally present at the ends of linear eukaryotic chromosomes (telomeres) and also produced in a programmed manner in order to induce recombination and genetic exchange (meiosis). DSB are efficiently repaired through multiple individual pathways of homologous or non-homologous recombination and although knowledge of recombination mechanisms has considerably advanced, the complex interrelationships and regulation of these pathways are far from being fully understood. A particularly striking example of the danger of inappropriate recombination is seen in the severe karyotypic and genomic instability in the presence of telomere dysfunction. Current knowledge of roles and mechanisms of recombination in plants will be presented through discussion of our recent results on somatic recombination pathways, responses to telomere dysfunction and the differing requirements for proteins of the Rad51 family in pairing of meiotic centromeric regions and chromosome arms. � Da Ines, O., Abe, K., Goubely, C., Gallego, M. E., & White, C. I. (2012). Differing Requirements for RAD51 and DMC1 in Meiotic Pairing of Centromeres and Chromosome Arms in Arabidopsis thaliana. PLoS Genetics, 8(4), e1002636. � Wei Wei, Zhaoqing Ba, Min Gao, Yang Wu, Yanting Ma, Simon Amiard, Charles I. White, Jannie Michaela Rendtlew Danielsen, Yun-Gui Yang and Yijun Qi. (2012). A Role for Small RNAs in DNA Double-Strand Break Repair. Cell 149: 101-112 � Amiard S, Depeiges A, Allain E, White CI, Gallego ME (2011) Arabidopsis ATM and ATR Kinases Prevent Propagation of Genome Damage Caused by Telomere Dysfunction. Plant Cell, 23:4254-65. � C. Charbonnel, E. Allain, M.E. Gallego and C. White. (2011). Kinetic analysis of post-replicative DSB repair pathways in Arabidopsis. DNA Repair, 10:611-619.

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Seminar Prof Charles White on Thursday, February 14 at 11 am
by Delphine Verspeel 04 Feb '13

04 Feb '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 04 Feb '13

04 Feb '13

Calendar Name: seminars Scheduled for: Monday, February 4 2013, 15:00 - 16:30 Event text: Dr John Ryals President CEO Metabolon Inc. Durham North Carolina USA Details: �global metabolomic analysis applied to plant systems� ABSTRACT Metabolon Inc. is a diagnostics and services company offering the industry's a leading biochemical profiling platform. Its patented platform provides a global analysis of complex biological samples for the discovery of markers and pathways associated with drug action and disease. Metabolon has developed the technology to quickly identify and measure all of the biochemicals in a biological sample through its proprietary global processing method. Through the generation and interpretation of data, this method provides a precise understanding of biochemical changes which may explain key biological changes in plant systems. (2011) A sister group contrast using untargeted global metabolomic analysis delineates the biochemical regulation underlying desiccation tolerance in Sporobolus stapfianus. The Plant cell 23, 1231-1248. (2012) The chloroplast triggers developmental reprogramming when MUTS HOMOLOG1 is suppressed in plants. Plant Physiology (2012). "Proteomics and Metabolomics of Arabidopsis Responses to Perturbation of Glucosinolate Biosynthesis." Molecular Plant.

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PSB event reminder
by contact＠psb.vib-ugent.be 30 Jan '13

30 Jan '13

Calendar Name: seminars Scheduled for: Wednesday, January 30 2013, 11:00 - 12:30 Event text: Dr Jens Boch Martin Luther University Halle-Wittenberg Institute of Biology Department of Genetics Halle (Saale) GERMANY Details: Postdoc Innovation seminar �TALEs and TALENs for targeted genome engineering and expression control� ABSTRACT The DNA-binding domain of TALEs (transcription activator-like effectors) from plant-pathogenic Xanthomonas bacteria has become an important tool for the programmable and specific targeting of DNA. Natural TALEs function as transcription factors in plant cells to support pathogen colonization of host plants. TALE proteins bind to DNA via near-identical tandem repeats of 34 amino acids. Each repeat recognizes one base in the target DNA sequence via repeat-variable diresidues (RVDs). The simple and modular repeat architecture allows rearrangement of TALE repeats to generate artificial TALEs with virtually any tailored DNA-binding specificity1. Highly specific genome-editing TALE nucleases (TALENs) can be engineered for targeted mutagenesis in plants and a wide variety of other eukaryotic organisms. We analyzed specificities and activities of TALEs experimentally in a transient reporter system using Agrobacterium-mediated expression in planta. Different RVDs exist in nature, but the DNA-specificity of only a few of them is known, so far. We will present novel results on RVD specificity and efficiency2, as well as the design of programmable gene switches and programmable precision mutagenesis tools. TALEs and TALENs are versatile virulence factors for the pathogen and exceptional tools for biotechnology. 1. Boch, J. et al. Science 326, 1509-1512 (2009). 2. Streubel et al. Nat. Biotechnol. 30, 593-595 (2012).

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Postdoc Innovation Seminar Dr Jens Boch on Wednesday, January 30 at 11am
by Delphine Verspeel 29 Jan '13

29 Jan '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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Seminar Dr John Ryals on Monday, February 4 at 3 pm in the Jozef Schell Seminar Room
by Delphine Verspeel 29 Jan '13

29 Jan '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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Seminar Prof Achim Walter on Monday, February 4 at 11 am
by Delphine Verspeel 28 Jan '13

28 Jan '13

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars . cid:946540515@15122008-0D1CPSBcid:946540515@15122008-0D23 VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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Upcoming seminars February
by Delphine Verspeel 28 Jan '13

28 Jan '13

Dear All, Enclosed you can find our upcoming seminars for February. Kind regards, Delphine ___________________________________________ Delphine Verspeel Administrative Assistant VIB, Department of Plant Systems Biology Ghent University Technologiepark 927 9052 Gent Belgium Phone: +32/9/33.13.800 Fax: +32/9/33.13.809 Http://www.psb.ugent.be despe(a)psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 28 Jan '13

28 Jan '13

Calendar Name: seminars Scheduled for: Wednesday, January 30 2013, 11:00 - 12:30 Event text: Dr Jens Boch Martin Luther University Halle-Wittenberg Institute of Biology Department of Genetics Halle (Saale) GERMANY Details: Postdoc Innovation seminar �TALEs and TALENs for targeted genome engineering and expression control� ABSTRACT The DNA-binding domain of TALEs (transcription activator-like effectors) from plant-pathogenic Xanthomonas bacteria has become an important tool for the programmable and specific targeting of DNA. Natural TALEs function as transcription factors in plant cells to support pathogen colonization of host plants. TALE proteins bind to DNA via near-identical tandem repeats of 34 amino acids. Each repeat recognizes one base in the target DNA sequence via repeat-variable diresidues (RVDs). The simple and modular repeat architecture allows rearrangement of TALE repeats to generate artificial TALEs with virtually any tailored DNA-binding specificity1. Highly specific genome-editing TALE nucleases (TALENs) can be engineered for targeted mutagenesis in plants and a wide variety of other eukaryotic organisms. We analyzed specificities and activities of TALEs experimentally in a transient reporter system using Agrobacterium-mediated expression in planta. Different RVDs exist in nature, but the DNA-specificity of only a few of them is known, so far. We will present novel results on RVD specificity and efficiency2, as well as the design of programmable gene switches and programmable precision mutagenesis tools. TALEs and TALENs are versatile virulence factors for the pathogen and exceptional tools for biotechnology. 1. Boch, J. et al. Science 326, 1509-1512 (2009). 2. Streubel et al. Nat. Biotechnol. 30, 593-595 (2012).

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