- Seminars - maillist.psb.ugent.be

Upcoming seminars April
by Delphine Verspeel 22 Mar '11

22 Mar '11

Dear All Enclosed you can find our upcoming seminars for April. Kind regards, Delphine ____________________________________________ Delphine Verspeel Administrative Assistant VIB, Department of Plant Systems Biology Ghent University Technologiepark 927 9052 Gent Belgium Phone: +32/9/33.13.800 Fax: +32/33.13.809 <Http://www.psb.ugent.be> Http://www.psb.ugent.be <mailto:nahau@psb.vib-ugent.be> despe(a)psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 17 Mar '11

17 Mar '11

Calendar Name: seminars Scheduled for: Thursday, March 17 2011, 11:00 - 12:30 Event text: Dr John Runions Oxford Brookes University School of Life Sciences Oxford UK Details: "Plant cell membrane protein dynamics" ABSTRACT Cell membrane proteins play a key role in the ability of plants to sense and respond to their environments. We have been utilizing live-cell imaging techniques to study protein interactions within and across the plasma membrane. A clue to the way in which membrane proteins associate with each other is in the way in which they diffuse within the plasma membrane. Using photoactivation and photobleaching of fluorescent proteins, we have been able to determine that different types of proteins have differing mobility and we are now working to determine how protein conformation and insertion into membranes govern mobility characteristics. I will illustrate the technique that we have developed by describing three recent case studies. Plants regulate plasma membrane viscosity by altering their fatty acid composition in response to temperature fluctuations. We have determined, however, by examining diffusion of the membrane marker LTI6b-GFP that these alterations maintain homeostasis of membrane viscosity. Secondly, the plasma membrane protein formin1 is an actin cytoskeleton regulatory protein that remains relatively immobile and we have determined that it is anchored through association with the cell wall. This is the first report of a protein bridging between the cytoskeleton and the cell wall. Finally, a new study with colleagues at VIB, Gent has revealed that lateral mobility of PIN proteins and ultimately their asymmetrical distribution in polarized cells is controlled by interactions with the extracellular matrix. These findings should be of general interest to physiologists, cell biologists and developmental biologists alike. Understanding the mechanisms of protein associations at the cell membrane will be key to new discoveries in these fields.

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PSB event reminder
by contact＠psb.vib-ugent.be 15 Mar '11

15 Mar '11

Calendar Name: seminars Scheduled for: Thursday, March 17 2011, 11:00 - 12:30 Event text: Dr John Runions Oxford Brookes University School of Life Sciences Oxford UK Details: "Plant cell membrane protein dynamics" ABSTRACT Cell membrane proteins play a key role in the ability of plants to sense and respond to their environments. We have been utilizing live-cell imaging techniques to study protein interactions within and across the plasma membrane. A clue to the way in which membrane proteins associate with each other is in the way in which they diffuse within the plasma membrane. Using photoactivation and photobleaching of fluorescent proteins, we have been able to determine that different types of proteins have differing mobility and we are now working to determine how protein conformation and insertion into membranes govern mobility characteristics. I will illustrate the technique that we have developed by describing three recent case studies. Plants regulate plasma membrane viscosity by altering their fatty acid composition in response to temperature fluctuations. We have determined, however, by examining diffusion of the membrane marker LTI6b-GFP that these alterations maintain homeostasis of membrane viscosity. Secondly, the plasma membrane protein formin1 is an actin cytoskeleton regulatory protein that remains relatively immobile and we have determined that it is anchored through association with the cell wall. This is the first report of a protein bridging between the cytoskeleton and the cell wall. Finally, a new study with colleagues at VIB, Gent has revealed that lateral mobility of PIN proteins and ultimately their asymmetrical distribution in polarized cells is controlled by interactions with the extracellular matrix. These findings should be of general interest to physiologists, cell biologists and developmental biologists alike. Understanding the mechanisms of protein associations at the cell membrane will be key to new discoveries in these fields.

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seminar Dr John Runions 17/03
by Nathalie Vanden Hautte 23 Feb '11

23 Feb '11

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars <https://maillist.psb.ugent.be/mailman/listinfo/seminars> . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars <https://maillist.psb.ugent.be/mailman/options/seminars> . VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 15 Feb '11

15 Feb '11

Calendar Name: seminars Scheduled for: Tuesday, February 15 2011, 11:00 - 12:30 Event text: Prof Herman H�fte Institut Jean-Pierre Bourgin UMR1318 INRA-AgroParisTech INRA Centre de Versailles-Grignon Versailles FRANCE Details: "The role of cell wall synthesis and modification in plant development" ABSTRACT In this presentation I first will show how the regulation of cellulose synthesis impacts on plant growth and morphogenesis. Next, I will discuss how the auxin-controlled modification of pectin in the shoot apical meristem leads to changes in tissue mechanics and the formation of organ primordia.

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PSB event reminder
by contact＠psb.vib-ugent.be 13 Feb '11

13 Feb '11

Calendar Name: seminars Scheduled for: Tuesday, February 15 2011, 11:00 - 12:30 Event text: Prof Herman H�fte Institut Jean-Pierre Bourgin UMR1318 INRA-AgroParisTech INRA Centre de Versailles-Grignon Versailles FRANCE Details: "The role of cell wall synthesis and modification in plant development" ABSTRACT In this presentation I first will show how the regulation of cellulose synthesis impacts on plant growth and morphogenesis. Next, I will discuss how the auxin-controlled modification of pectin in the shoot apical meristem leads to changes in tissue mechanics and the formation of organ primordia.

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seminar Prof Herman Höfte 15/02
by Nathalie Vanden Hautte 11 Feb '11

11 Feb '11

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars <https://maillist.psb.ugent.be/mailman/listinfo/seminars> . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars <https://maillist.psb.ugent.be/mailman/options/seminars> . VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 10 Feb '11

10 Feb '11

Calendar Name: seminars Scheduled for: Thursday, February 10 2011, 11:00 - 12:30 Event text: Dr Renier Van der Hoorn Chemical Genomics Centre of the Max Planck Society Max Planck Institute for Plant Breeding Research Cologne GERMANY Details: "Mining the active proteome from plant-pathogen interactions" ABSTRACT The activity of proteins in proteomes and living organisms can be displayed with Activity-based Protein Profiling (ABPP). ABPP is a technology that makes use of small molecule probes that label the active site of enzyme classes in an activity-dependent manner. Labeling is covalent and irreversible and facilitates the separation and detection of labeled proteins using protein gels and the purification and identification by mass spectrometry. This method displays functional proteomic information, which contrasts strongly with traditional transcriptomic and proteomic datasets, which are poor predictors on the functional state of proteins.The introduction and application of ABPP in plant science is the mission of the Plant Chemetics laboratory. We have validated and introduced activity-based probes for papain-like cysteine proteases, vacuolar processing enzymes, serine hydrolases, lipases, acyltransferases and the proteasome. Probes for kinases, glycosidases and other enzymes are being developed and tested. Procedures for in vivo labeling and imaging have been established. Studies of plant-pathogen interactions using these probes have revealed that the proteasome and Cys proteases are activated during immune responses, and suppressed during infection by successful pathogens. These examples illustrate the use of ABPP to study protein activities in any organism to address many different biological questions.

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interested in update seminars
by Kris Audenaert 08 Feb '11

08 Feb '11

kris.audenaert(a)hogent.be

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PSB event reminder
by contact＠psb.vib-ugent.be 08 Feb '11

08 Feb '11

Calendar Name: seminars Scheduled for: Thursday, February 10 2011, 11:00 - 12:30 Event text: Dr Renier Van der Hoorn Chemical Genomics Centre of the Max Planck Society Max Planck Institute for Plant Breeding Research Cologne GERMANY Details: "Mining the active proteome from plant-pathogen interactions" ABSTRACT The activity of proteins in proteomes and living organisms can be displayed with Activity-based Protein Profiling (ABPP). ABPP is a technology that makes use of small molecule probes that label the active site of enzyme classes in an activity-dependent manner. Labeling is covalent and irreversible and facilitates the separation and detection of labeled proteins using protein gels and the purification and identification by mass spectrometry. This method displays functional proteomic information, which contrasts strongly with traditional transcriptomic and proteomic datasets, which are poor predictors on the functional state of proteins.The introduction and application of ABPP in plant science is the mission of the Plant Chemetics laboratory. We have validated and introduced activity-based probes for papain-like cysteine proteases, vacuolar processing enzymes, serine hydrolases, lipases, acyltransferases and the proteasome. Probes for kinases, glycosidases and other enzymes are being developed and tested. Procedures for in vivo labeling and imaging have been established. Studies of plant-pathogen interactions using these probes have revealed that the proteasome and Cys proteases are activated during immune responses, and suppressed during infection by successful pathogens. These examples illustrate the use of ABPP to study protein activities in any organism to address many different biological questions.

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