- Seminars - maillist.psb.ugent.be

PSB event reminder
by contact＠psb.vib-ugent.be 19 Sep '10

19 Sep '10

Calendar Name: seminars Scheduled for: Tuesday, September 21 2010, 15:00 - 16:30 Event text: Toshihiro Obata Max-Planck-Institute f�r Molecular Plant Physiology Potsdam-Golm GERMANY Details: "Metabolic response to oxidative stress in Arabidopsis" ABSTRACT Plants must reconfigure their metabolic network under stress conditions to prevent the accumulation of reactive oxygen species (ROS) and to produce indispensable metabolites. We have investigated the response of Arabidopsis roots to menadione-induced oxidative stress by means of metabolite profiling and transcriptome analysis. The results suggested the importance of mitochondrial metabolism in stress response and also highlighted a complex relationship between the levels of transcripts, metabolites, and metabolic flux. We hypothesise the reassembly of enzyme protein complexes as a molecular mechanism of mitochondrial metabolic regulation. A proteomic approach using blue-native/SDS-PAGE revealed that some mitochondrial enzymes associated to or dissociated from a protein complex under oxidative stress. These results suggest the regulation of the TCA cycle, glycolysis, ascorbate and amino acid metabolism under oxidative stress by reassembly of enzyme supercomplexes.

1 0

PSB event reminder
by contact＠psb.vib-ugent.be 19 Sep '10

19 Sep '10

Calendar Name: seminars Scheduled for: Tuesday, September 21 2010, 11:00 - 12:30 Event text: Alisdair R. Fernie Max-Planck Institute f�r Molecular Plant Physiology Potsdam-Golm GERMANY Details: "Metabolomics from fundamental understanding to marker-assisted breeding" ABSTRACT Metabolomics approaches enable the parallel assessment of the levels of a broad range of metabolites and have been documented to have great value in both phenotyping and diagnostic analyses in plants. These tools have recently been turned to evaluation of the natural variance apparent in metabolite composition. Here, I will describe exciting progress made in the identification of the genetic determinants of crop plant chemical composition, focussing on the application of metabolomics strategies and their integration with other high-throughput technologies. The Zamir Solanum pennellii introgression lines will be used as a case study and I will illustrate the value of this approach by demonstrating a few examples whereby we have been able to identify the genetic factors underlying important agronomic and nutritional traits. Whilst work in this area is currently carried out at the level of fundamental science I will conclude with an outlook of my personal perspective of its applied uses.

1 0

seminar Alisdair Fernie
by Nathalie Vanden Hautte 14 Sep '10

14 Sep '10

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars <https://maillist.psb.ugent.be/mailman/listinfo/seminars> . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars <https://maillist.psb.ugent.be/mailman/options/seminars> . VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

1 0

PSB event reminder
by contact＠psb.vib-ugent.be 06 Sep '10

06 Sep '10

Calendar Name: seminars Scheduled for: Monday, September 6 2010, 11:00 - 12:30 Event text: Prof Maria Helena S. Goldman Department of Biology, FFCLRP, University of S�o Paulo BRAZIL Details: "SCI1, A TISSUE-SPECIFIC CELL CYCLE REGULATOR THAT CONTROLS STIGMA/STYLE DEVELOPMENT" ABSTRACT The success of plant reproduction depends on the appropriate development of the reproductive organs, which involves general and specific regulatory networks. We have characterized a gene, SCI1 (stigma/style cell cycle inhibitor 1), encoding a nuclear small lysine-rich protein that exerts an inhibitory action on cell cycle. qRT-PCR and in situ hybridization experiments showed that SCI1 is stigma/style-specific and developmentally regulated. SCI1 RNAi knockdown and overexpression transgenic plants exhibited stigmas/styles with remarkably enlarged and reduced areas, respectively, due to differences in cell numbers. Alterations on SCI1 expression affected cell divisions on the stigmatic secretory zone (SSZ) and the differentiation timing of the papillar cells. Taken together, our results reveal that SCI1 is a novel tissue-specific negative cell cycle regulator that couples stigma cell division and differentiation. Additionally, two-hybrid and BiFC experiments showed that SCI1 interacts with A-type cyclins. We conclude that SCI1 represents a novel developmentally regulated tissue-specific gene that controls cell proliferation/differentiation, probably as a component of a signaling pathway involved in upper pistil development.

1 0

PSB event reminder
by contact＠psb.vib-ugent.be 04 Sep '10

04 Sep '10

Calendar Name: seminars Scheduled for: Monday, September 6 2010, 11:00 - 12:30 Event text: Prof Maria Helena S. Goldman Department of Biology, FFCLRP, University of S�o Paulo BRAZIL Details: "SCI1, A TISSUE-SPECIFIC CELL CYCLE REGULATOR THAT CONTROLS STIGMA/STYLE DEVELOPMENT" ABSTRACT The success of plant reproduction depends on the appropriate development of the reproductive organs, which involves general and specific regulatory networks. We have characterized a gene, SCI1 (stigma/style cell cycle inhibitor 1), encoding a nuclear small lysine-rich protein that exerts an inhibitory action on cell cycle. qRT-PCR and in situ hybridization experiments showed that SCI1 is stigma/style-specific and developmentally regulated. SCI1 RNAi knockdown and overexpression transgenic plants exhibited stigmas/styles with remarkably enlarged and reduced areas, respectively, due to differences in cell numbers. Alterations on SCI1 expression affected cell divisions on the stigmatic secretory zone (SSZ) and the differentiation timing of the papillar cells. Taken together, our results reveal that SCI1 is a novel tissue-specific negative cell cycle regulator that couples stigma cell division and differentiation. Additionally, two-hybrid and BiFC experiments showed that SCI1 interacts with A-type cyclins. We conclude that SCI1 represents a novel developmentally regulated tissue-specific gene that controls cell proliferation/differentiation, probably as a component of a signaling pathway involved in upper pistil development.

1 0

PSB event reminder
by contact＠psb.vib-ugent.be 26 Aug '10

26 Aug '10

Calendar Name: seminars Scheduled for: Thursday, August 26 2010, 11:00 - 12:30 Event text: Prof Ian Small Director, ARC Centre of Excellence in Plant Energy Biology University of Western Australia AUSTRALIA Details: "Perfectly Prepared RNA: getting transcripts ready for translation" ABSTRACT Ian Small ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley 6009 WA, Australia Organelle gene expression involves a series of more or less coupled processes from transcription through transcript processing to translation. Many of the events that occur during these processes require sequence recognition by RNA processing factors. It has become abundantly clear over the last decade that in plants, pentatricopeptide repeat (PPR) proteins are the most numerous of these factors, with a bewildering array of 450+ family members in most angiosperms studied to date. The development of screens for RNA processing defects has enabled the identification of the functions of some of these proteins by reverse genetics; several other PPR genes have been singled out by forward genetic screens for phenotypes that involve defects in organelle function. The RNA targets and molecular functions for over 40 PPR proteins have been characterised, allowing the first generalisations to be drawn concerning their modes of action. Although the functions of over 90% of the PPR family are still to be worked out, it is becoming clear that certain subclasses of the family have a strong tendency to be associated with particular molecular processes such as transcription, splicing or RNA editing. Progress is being made at modelling the structure of PPR proteins and understanding how they recognise specific RNA sequences, offering hope that they could form the basis for a new way of designing custom-made RNA binding proteins for biotechnology

1 0

upcoming seminars September
by Nathalie Vanden Hautte 24 Aug '10

24 Aug '10

Dear All Enclosed you can find our upcoming seminars for September. Kind regards Nathalie ____________________________________________ Nathalie Vanden Hautte Administrative Assistant VIB, Department of Plant Systems Biology Ghent University Technologiepark 927 9052 Gent Belgium Phone: +32/9/33.13.800 Fax: +32/33.13.809 Http://www.psb.ugent.be nahau(a)psb.vib-ugent.be

1 0

PSB event reminder
by contact＠psb.vib-ugent.be 24 Aug '10

24 Aug '10

Calendar Name: seminars Scheduled for: Thursday, August 26 2010, 11:00 - 12:30 Event text: Prof Ian Small Director, ARC Centre of Excellence in Plant Energy Biology University of Western Australia AUSTRALIA Details: "Perfectly Prepared RNA: getting transcripts ready for translation" ABSTRACT Ian Small ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley 6009 WA, Australia Organelle gene expression involves a series of more or less coupled processes from transcription through transcript processing to translation. Many of the events that occur during these processes require sequence recognition by RNA processing factors. It has become abundantly clear over the last decade that in plants, pentatricopeptide repeat (PPR) proteins are the most numerous of these factors, with a bewildering array of 450+ family members in most angiosperms studied to date. The development of screens for RNA processing defects has enabled the identification of the functions of some of these proteins by reverse genetics; several other PPR genes have been singled out by forward genetic screens for phenotypes that involve defects in organelle function. The RNA targets and molecular functions for over 40 PPR proteins have been characterised, allowing the first generalisations to be drawn concerning their modes of action. Although the functions of over 90% of the PPR family are still to be worked out, it is becoming clear that certain subclasses of the family have a strong tendency to be associated with particular molecular processes such as transcription, splicing or RNA editing. Progress is being made at modelling the structure of PPR proteins and understanding how they recognise specific RNA sequences, offering hope that they could form the basis for a new way of designing custom-made RNA binding proteins for biotechnology

1 0

seminar Prof Maria-Helana Goldman 06/09
by Nathalie Vanden Hautte 23 Aug '10

23 Aug '10

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars <https://maillist.psb.ugent.be/mailman/listinfo/seminars> . If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/options/seminars <https://maillist.psb.ugent.be/mailman/options/seminars> . VIB - Department of Plant Systems Biology Technologiepark 927 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 Http://www.psb.vib-ugent.be

1 0

upcoming seminars September
by Nathalie Vanden Hautte 23 Aug '10

23 Aug '10

Dear All Enclosed you can find our upcoming seminars for September. Kind regards Nathalie ____________________________________________ Nathalie Vanden Hautte Administrative Assistant VIB, Department of Plant Systems Biology Ghent University Technologiepark 927 9052 Gent Belgium Phone: +32/9/33.13.800 Fax: +32/33.13.809 Http://www.psb.ugent.be nahau(a)psb.vib-ugent.be

1 0