[Confocal] Leica Sp2: 514 laser is unstable, sometimes it works, sometimes not. Other lasers are fine.
Dear confocal lasers, The 514 laser from the Leica SP2 gives unstable output. Sometimes working nicely, other times not. The other lasers are working nicely. Depending on your dye or fluorophore it could be possible to excite your sample by using another laserline. Leica SP2 has also a 496 laser which is the closest to the 514 laser. The company is informed and I will keep you updated. Best regards, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
Solved! Enjoy imaging! Don't hesitate to contact Daniel or me in case of problems. Gr, Evelien
From: "Evelien Mylle" <evmyl@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Tuesday, March 13, 2018 12:46:03 PM Subject: [Confocal] Leica Sp2: 514 laser is unstable, sometimes it works, sometimes not. Other lasers are fine.
Dear confocal lasers,
The 514 laser from the Leica SP2 gives unstable output. Sometimes working nicely, other times not. The other lasers are working nicely. Depending on your dye or fluorophore it could be possible to excite your sample by using another laserline. Leica SP2 has also a 496 laser which is the closest to the 514 laser.
The company is informed and I will keep you updated.
Best regards,
Evelien
Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
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Evelien Mylle