[Confocal] Leica SP8: bidirectional scanning requires phase correction
Dear Leica SP8 users, Since the upgrade of the software you need to adapt the phaseX correction when doing the bidirectional scanning (see attach how to do). Before the upgrade this was (mostly) never needed, but now this is the case. Without adaptation you get a very strange image (there is a shift from the startposition for each line compared to the previous line, it seems a bit like a splitted image). (when you see it you will notice that it is not good!) There is no fixed value to where you need to change, but probably it is in the range of 20. Look at the image till it looks nice. I will contact the company to ask if we can go back to the situation where no adaptation is required. This information is also available on following folder: [ file://psb.ugent.be/shares/research/images/images_cameras/confocal/Problems_Leica%20SP8/MAIL%20EVMYL | \\psb.ugent.be\shares\research\images\images_cameras\confocal\Problems_Leica SP8\MAIL EVMYL ] In case of problems, don't hesitate to contact Daniel or me. Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
Dear Leica SP8 users, The phaseX correction is adapted to a default value of 24.08 (in stead of the -41). With this value in most of the cases you do NOT need adaptation of the phase X correction value when doing bidirectional scanning. (So, we are back in the situation as before the upgrade). In case of problems, don't hesitate to contact Daniel or me. Gr, Evelien
From: "Evelien Mylle" <evmyl@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Friday, March 30, 2018 3:52:11 PM Subject: [Confocal] Leica SP8: bidirectional scanning requires phase correction
Dear Leica SP8 users,
Since the upgrade of the software you need to adapt the phaseX correction when doing the bidirectional scanning (see attach how to do).
Before the upgrade this was (mostly) never needed, but now this is the case.
Without adaptation you get a very strange image (there is a shift from the startposition for each line compared to the previous line, it seems a bit like a splitted image). (when you see it you will notice that it is not good!) There is no fixed value to where you need to change, but probably it is in the range of 20. Look at the image till it looks nice. I will contact the company to ask if we can go back to the situation where no adaptation is required.
This information is also available on following folder:
[ file://psb.ugent.be/shares/research/images/images_cameras/confocal/Problems_Leica%20SP8/MAIL%20EVMYL | \\psb.ugent.be\shares\research\images\images_cameras\confocal\Problems_Leica SP8\MAIL EVMYL ]
In case of problems, don't hesitate to contact Daniel or me.
Gr,
Evelien
Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
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Evelien Mylle