You are invited to attend “Tinkering with Meiosis: To Mix, or Not to Mix“ ON 16 March 2020

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at https://maillist.psb.ugent.be/mailman/listinfo/seminars. “Tinkering with Meiosis: To Mix, or Not to Mix“ Prof Raphael Mercier Department of Chromosome Biology Max Planck Institute for Plant Breeding Research (MPIPZ) Cologne GERMANY Monday, March 16, 2020 10:00 Jozef Schell seminar room Technologiepark 71 - 9052 Zwijnaarde View event details here Invited by Prof Dirk Inzé Prof Lieven De Veylder and Dr Erik van der Biezen If you do not wish to receive this information anymore, please unsubscribe from future mailings at https://maillist.psb.ugent.be/mailman/listinfo/seminars VIB - UGent Center for Plant Systems Biology Technologiepark 71 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 http://www.psb.vib-ugent.be

Dear All, The seminar of Prof Raphael Mercier on Monday, March 16 is canceled. Our apologies for this inconvenience. Kind regards, Delphine Van: "Delphine Verspeel" <despe@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Maandag 2 maart 2020 15:41:23 Onderwerp: You are invited to attend “Tinkering with Meiosis: To Mix, or Not to Mix“ ON 16 March 2020 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . “Tinkering with Meiosis: To Mix, or Not to Mix“ Prof Raphael Mercier Department of Chromosome Biology Max Planck Institute for Plant Breeding Research (MPIPZ) Cologne GERMANY Monday, March 16, 2020 10:00 Jozef Schell seminar room Technologiepark 71 - 9052 Zwijnaarde View event details [ https://www.psb.ugent.be/index.php?option=com_jevents&task=icalrepeat.detail&evid=313&Itemid=971&year=2020&month=03&day=02&title=tinkering-with-meiosis-to-mix-or-not-to-mix&uid=02e0ad65006a6a3bdcedce46e0d59f1e | here ] Invited by Prof Dirk Inzé Prof Lieven De Veylder and Dr Erik van der Biezen If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] VIB - UGent Center for Plant Systems Biology Technologiepark 71 B - 9052 Gent/Zwijnaarde Phone: +32 9 33 13 800 Fax: +32 9 33 13 809 [ http://www.psb.vib-ugent.be/ | http://www.psb.vib-ugent.be ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . ''Deciphering the role of brassinosteroids in plant adaptation to climate change'' Dr Ana Caño Centre for Research in Agricultural Genomics (CRAG) Barcelona SPAIN Wednesday, March 30, 2022 11:00 Abstract to be announced Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Jenny Russinova If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . ''Maize reproduction: Focus on pollen and seed development'' Dr Thomas Widiez Plant Reproduction and Development ENS Lyon FRANCE Thursday, March 31, 2022 11:00 In flowering plants, sexual reproduction is characterized by a unique biological process called double fertilization. It consists of two synchronized fusion events between male and female gametes: two sperm cells (from the same pollen grain) fused separately with the two female gametes: egg cell and central cell. This double fertilization gives rise to two different individuals/tissues: the embryo and the endosperm, which are enclosed by maternal tissues. A coordinated development of these three genetically distinct tissues, organized like a Russian doll, lead to the seed. By using maize and combining OMICs, genetics (mutants and CRISPR/cas9) and cell biology approaches, our work aims to tackle questions on plant reproduction: How is the synchronization of double fertilization ensured? How is the communication between the three seed compartments established to ensure proper seed development? I will illustrate our research focusing on two aspects: (1) On the pollen side, I will present the characterization of a still enigmatic interface: the endo-plasma membrane (endo-PM) that originates from the pollen vegetative cell and wraps the two sperm cells, forming a unique “cell within a cell” structure. (2) On the seed development side, I will present the characterization of a new embryo/endosperm interface: the endosperm adjacent to scutellum (EAS). Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Moritz Nowack If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Dinsdag 15 maart 2022 13:29:04 Onderwerp: You are invited to attend “Maize reproduction: Focus on pollen and seed development” ON March 31, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . ''Maize reproduction: Focus on pollen and seed development'' Dr Thomas Widiez Plant Reproduction and Development ENS Lyon FRANCE Thursday, March 31, 2022 11:00 In flowering plants, sexual reproduction is characterized by a unique biological process called double fertilization. It consists of two synchronized fusion events between male and female gametes: two sperm cells (from the same pollen grain) fused separately with the two female gametes: egg cell and central cell. This double fertilization gives rise to two different individuals/tissues: the embryo and the endosperm, which are enclosed by maternal tissues. A coordinated development of these three genetically distinct tissues, organized like a Russian doll, lead to the seed. By using maize and combining OMICs, genetics (mutants and CRISPR/cas9) and cell biology approaches, our work aims to tackle questions on plant reproduction: How is the synchronization of double fertilization ensured? How is the communication between the three seed compartments established to ensure proper seed development? I will illustrate our research focusing on two aspects: (1) On the pollen side, I will present the characterization of a still enigmatic interface: the endo-plasma membrane (endo-PM) that originates from the pollen vegetative cell and wraps the two sperm cells, forming a unique “cell within a cell” structure. (2) On the seed development side, I will present the characterization of a new embryo/endosperm interface: the endosperm adjacent to scutellum (EAS). Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Moritz Nowack If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' In search of the roots of roots: 400 million years of plant root evolution '' Dr Sandy Hetherington Institute of Molecular Plant Sciences School of Biological Sciences University of Edinburgh UNITED KINGDOM Thursday, April 21, 2022 11:00 The evolution of plant roots shaped the terrestrial landscape and underpinned the radiation of land plants. The aim of the talk is to provide an overview of the origin and early diversification of plant roots. Fossils provide a unique window into early root evolution and it is only by combing evidence from the fossil record with the diversity of living rooting systems that we can piece together the evolution of plant roots over their 400 million year history. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé, Prof Tom Beeckman and Dr Hans Motte If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Vein Patterning by Coordinated Polar Cell-Behavior '' Prof Enrico Scarpella Department of Biological Sciences University of Alberta CANADA Thursday, April 28, 2022 11:00 To form tissue networks, animal cells migrate along specific directions and interact through proteins protruding from specific segments of their plasma membranes. Plant cells can do neither, yet plants form vein networks. How plants do so is unclear, but the prevailing hypothesis proposes that GNOM — a regulator of membrane trafficking — positions PIN-FORMED auxin transporters to specific segments of the plasma membrane; the resulting cell-to-cell, polar transport of auxin would induce vein formation. Contrary to predictions of the hypothesis, we find that vein formation occurs in the absence of PIN-FORMED auxin transporters; that the residual auxin-transport-independent vein-patterning activity relies on auxin signaling; and that a GNOM -dependent signal acts upstream of both auxin transport and auxin signaling to induce vein formation. Nevertheless, plants inhibited in both auxin transport and auxin signaling still form veins. Patterning of vascular cells into veins is instead prevented in the absence of GNOM function, suggesting the existence of at least one more GNOM -dependent vein-patterning pathway. Our fundings suggest that such a pathway depends on the movement of an auxin-dependent signal through the plasmodesmata (PDs) intercellular channels. PD permeability is high where veins are forming, lowers between veins and nonvascular tissues, but remains high between vein cells. Impaired ability to regulate PD aperture leads to defects in auxin transport and signaling, ultimately leading to vein patterning defects that are enhanced by inhibition of auxin transport or signaling. GNOM controls PD aperture regulation, and simultaneous inhibition of auxin signaling, auxin transport, and regulated PD aperture phenocopies loss of GNOM function. Therefore, veins are patterned by the coordinated action of three GNOM -dependent pathways: auxin signaling, polar auxin transport, and movement of an auxin-dependent signal through PDs. Such control is an unprecedented mechanism of tissue network formation in multicellular organisms. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Bert De Rybel If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Vrijdag 8 april 2022 16:30:24 Onderwerp: You are invited to attend “Vein Patterning by Coordinated Polar Cell-Behavior” ON April 28, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Vein Patterning by Coordinated Polar Cell-Behavior '' Prof Enrico Scarpella Department of Biological Sciences University of Alberta CANADA Thursday, April 28, 2022 11:00 To form tissue networks, animal cells migrate along specific directions and interact through proteins protruding from specific segments of their plasma membranes. Plant cells can do neither, yet plants form vein networks. How plants do so is unclear, but the prevailing hypothesis proposes that GNOM — a regulator of membrane trafficking — positions PIN-FORMED auxin transporters to specific segments of the plasma membrane; the resulting cell-to-cell, polar transport of auxin would induce vein formation. Contrary to predictions of the hypothesis, we find that vein formation occurs in the absence of PIN-FORMED auxin transporters; that the residual auxin-transport-independent vein-patterning activity relies on auxin signaling; and that a GNOM -dependent signal acts upstream of both auxin transport and auxin signaling to induce vein formation. Nevertheless, plants inhibited in both auxin transport and auxin signaling still form veins. Patterning of vascular cells into veins is instead prevented in the absence of GNOM function, suggesting the existence of at least one more GNOM -dependent vein-patterning pathway. Our fundings suggest that such a pathway depends on the movement of an auxin-dependent signal through the plasmodesmata (PDs) intercellular channels. PD permeability is high where veins are forming, lowers between veins and nonvascular tissues, but remains high between vein cells. Impaired ability to regulate PD aperture leads to defects in auxin transport and signaling, ultimately leading to vein patterning defects that are enhanced by inhibition of auxin transport or signaling. GNOM controls PD aperture regulation, and simultaneous inhibition of auxin signaling, auxin transport, and regulated PD aperture phenocopies loss of GNOM function. Therefore, veins are patterned by the coordinated action of three GNOM -dependent pathways: auxin signaling, polar auxin transport, and movement of an auxin-dependent signal through PDs. Such control is an unprecedented mechanism of tissue network formation in multicellular organisms. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Bert De Rybel If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Vrijdag 1 april 2022 18:22:59 Onderwerp: You are invited to attend “In search of the roots of roots: 400 million years of plant root evolution” ON April 21, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' In search of the roots of roots: 400 million years of plant root evolution '' Dr Sandy Hetherington Institute of Molecular Plant Sciences School of Biological Sciences University of Edinburgh UNITED KINGDOM Thursday, April 21, 2022 11:00 The evolution of plant roots shaped the terrestrial landscape and underpinned the radiation of land plants. The aim of the talk is to provide an overview of the origin and early diversification of plant roots. Fossils provide a unique window into early root evolution and it is only by combing evidence from the fossil record with the diversity of living rooting systems that we can piece together the evolution of plant roots over their 400 million year history. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé, Prof Tom Beeckman and Dr Hans Motte If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Avoiding unnecessary stress: post-transcriptional regulation of mitochondrial dysfunction responses in plants '' Associate Prof Saijaliisa Kangasjärvi Faculty of Biological and Environmental Sciences University of Helsinki FINLAND Thursday, May 12, 2022 11:00 Saijaliisa Kangasjärvi 1,2,3 1 Faculty of Biological and Environmental Sciences, Organismal and Evolutionary Biology Research Programme, University of Helsinki, Finland. 2 Faculty of Agriculture and Forestry, Department of Agricultural Sciences, University of Helsinki, Finland. 3 Viikki Plant Science Center, University of Helsinki, Finland Organellar retrograde signals are vital in determining stress reactions in plants. We have identified Protein Phosphatase 2A (PP2A) as a cytosolic factor that controls organelle-induced stress reactions and pathogenesis responses in Arabidopsis thaliana. PP2A regulatory subunit B’γ (PP2A-B’γ) is required to maintain growth and prevent premature senescence under favorable conditions. On a molecular level, PP2A-B’γ controls multiple phosphoproteins that are elicited by mitochondrial dysfunction and critical in determining metabolic activities and detoxification capacity in plant cells. Under stress, the promoter of PP2A-B’γ becomes transiently inactivated by mitochondrial dysfunction signals. Hence, PP2A-B’γ does not prevent stress responses upon environmental challenges, but is an important regulator of stress recovery. Phosphoproteomic analysis suggests networks related to plant immunity, vesicle transport and RNA metabolism as regulation targets for PP2A-B’γ. PP2A-B’γ as a post-transcriptional regulator of mitochondrial dysfunction responses and stress recovery will be presented. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Frank Van Breusegem If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Plasma Membrane to Proline Metabolism: Signaling and metabolic mechanisms that control plant growth and drought resistance '' Prof Paul E. Verslues Institute of Plant and Microbial Biology Academia Sinica Taipei TAIWAN Monday, May 30, 2022 11:00 In this seminar Prof Paul E. Verslues will present two ongoing projects in our efforts to understand how plants adjust their growth and metabolism in response to moderate severity low water potential (drought) stress. While this type of stress typically does not lead to death of the plant, how the plant responds influences agronomic productivity and ability to adapt to changing environmental conditions. To better understand the role of proline metabolism in drought resistance and its regulation, we performed a forward genetic screen using a reporter driven by the promoter of Proline Dehydrogenase 1 ( ProDH1 pro :LUC; Shinde et al., 2016) and identified a low water potential stress hypersensitive mutant having a single amino acid change in the Non-Phototrophic Hypocotyl3 (NPH3)-domain of an NRL protein. The hypersensitive phenotype could be alleviated by blocking proline metabolism . The nrl point mutation disrupts NRL interaction with RAB small GTPases which are involved in endo-membrane trafficking. These results have implications for both stress biology and plant signaling. For stress biology, our results show that high levels of proline accumulation often seen during drought have the potential to elicit cell death responses unless specific mechanisms are activated to allow proline to accumulate safely. In terms of signaling, our results indicate that the NPH3 domain has previously unknown functions related to membrane trafficking which will enlighten research on the role of other NPH3 proteins. The plasma membrane-localized Clade E Growth-regulating (EGR) type 2C protein phosphatases and their target protein MASP1 have opposing effects on growth during drought stress (Bhaskara et al., 2017). EGRs and MASP1 have opposing gradients of expression across the root meristem and opposing effects on root meristem size at low water potential. Ectopic MASP1 or EGR expression increased or decreased, respectively, root meristem size and root elongation during low water potential stress. However, ectopic EGR expression had no effect on unstressed plants. Phosphomimic MASP1 could overcome EGR suppression of root meristem size. Together these results indicated that during low water potential leads to EGR activation and attenuation of MASP1 phosphorylation to regulate meristem size and activity (Longkumer et al., 2022). References Bhaskara GB, Wen T-N, Nguyen TT, Verslues PE (2017) Protein Phosphatase 2Cs and Microtubule-Associated Stress Protein 1 control microtubule stability, plant growth, and drought response. Plant Cell 29: 169-191 Longkumer T, Chen C-Y, Biancucci M, Bhaskara GB, Verslues PE (2022) Spatial differences in stoichiometry of EGR phosphatase and Microtubule-Associated Stress Protein 1 control root meristem activity during drought stress. Plant Cell : 34: 742–758 Shinde S, Villamor JG, Lin W-D, Sharma S, Verslues PE (2016) Proline coordination with fatty acid synthesis and redox metabolism of chloroplast and mitochondria. Plant Physiology 172: 1074-1088 . Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Hilde Nelissen If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Brassinosteroid gene regulatory networks at cellular resolution '' Dr Trevor Nolan Duke University and Howard Hughes Medical Institute Department of Biology Durham USA Thursday, June 2, 2022 11:00 Brassinosteroids (BRs) are a group of plant steroid hormones that regulate diverse processes such as cell division, cell elongation, and differentiation by controlling the activities of BES1 and BZR1 family transcription factors, which in turn mediate the expression of thousands of genes. The gene regulatory networks (GRNs) that control the diverse processes regulated by BRs are only partially understood, but likely involve additional context-specific regulatory factors. The Arabidopsis root is a tractable model to investigate these networks due to its simple organization and defined cell lineages. We recently constructed an integrated atlas of more than 110,000 cells using single-cell RNA-seq, which highlighted the continuous nature of development in the Arabidopsis root and provided new insights into cell identity acquisition (Shahan, Hsu, et al., 2022, https://doi.org/10.1016/j.devcel.2022.01.008). To further define spatiotemporal BR responses, we performed a time series single-cell RNA-seq experiment following BR treatment. Annotation enabled by our reference atlas allowed us to query BR-regulated gene expression among the majority of cell types and developmental stages of the root. We recovered known hotspots for BR signaling including the epidermis. Our data also indicate that BRs strongly influence gene expression in the cortex, especially in the elongation zone. Waddington-OT reconstruction of cortex trajectories showed that BRs trigger a shift from proliferation to elongation which is associated with increased expression of cell wall-related genes. Accordingly, loss of BR signaling in the cortex has little effect on meristem cell length but impairs cell expansion in the elongation zone. To discover regulators in the elongating cortex, we used CellOracle to infer GRN configurations across each cell type, developmental stage, and time point of our BR time series. Our GRNs and subsequent experimental analysis revealed two transcription factors that play a prominent role in cortex GRNs and affect BR-mediated cell elongation. Finally, we used scRNA-seq to define cell-type-specific changes in gene expression associated with reduced elongation in the cortex of our transcription factor mutants. When combined, these datasets represent more than 200,000 single-cell transcriptomes, providing a view of brassinosteroid-mediated gene expression at unprecedented resolution. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Jenny Russinova If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Challenges and Opportunities of Breeding by Design '' Fred Van Ex Vice President R&D Managing Director Inari Agriculture BELGIUM Thursday, June 16, 2022 11:00 Global demand for food and feed is increasing at a rapid pace as a consequence of a growing global population and challenging environmental conditions. Conventional and modern breeding approaches, developed during the Green Revolution, have had a major contribution to the consistent yield increases observed during the past decades but are leveling off and are no longer keeping pace with the growing demands. Reducing genetic diversity and lengthy breeding timelines will further limit what can be achieved through regular breeding. Rapid climate change is putting an additional challenge to what can be achieved in the timelines needed for breeding to obtain high yielding varieties adapted to new environmental conditions and concerns over environmental sustainability will reduce yield gains caused by use of agronomical inputs such as fertilizers and herbicides. Consequently, rapid and significant improvement of varieties need to be achieved through a combination of breeding, improved agronomical practices and new breeding techniques such as gene editing. In this seminar, an overview will be provided of the Inari Agriculture toolbox which is being developed. This toolbox is enabling us to develop improved crop varieties that deliver superior yields with reduced needs of inputs such as water and fertilizer in order to meet the global demands of food security and environmental sustainability. Our toolbox will enable the rapid development of novel allele variation by generating high order multiplex edited populations. Phenotypic selection of plants from this population enables the identification of novel, high order, allele combinations. In addition, the efficient generation of multitype edited plants leads to the generation of novel alleles. This approach already resulted in the identification of interesting genes and alleles linked with improved water and nitrogen use efficiency. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Multiple functions of the plant unique ESCRT component FREE1 in Arabidopsis thaliana '' Prof Liwen Jiang Shool of Life Sciences Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong (CUHK) CHINA Thursday, June 23, 2022 14:00 The plant endomembrane system contains several functionally distinct membrane-enclosed organelles, including the endoplasmic reticulum (ER), Golgi apparatus, trans-Golgi network (TGN) or early endosomes (EE), prevacuolar compartment (PVC) or multivesicular body (MVB) and vacuole. The endosomal sorting complex required for transport (ESCRT) machinery mediates the formation of intraluminal vesicles (ILVs) in MVBs as well as the sorting of the ubiquitinated cargoes into the internal vesicles (ILVs) in eukaryotes. Since 2014, we have characterized a plant unique ESCRT component termed FYVE domain protein required for endosomal sorting 1 (FREE1) and demonstrated that FEFF1 plays multiple functions with distinct underlying mechanisms in plants, including regulation of MVB and vacuole biogenesis (Current Biology 2014), crosstalk of the endomembrane system with the autophagic pathway (PNAS 2015), Abscisic Acid (ABA) signaling (Nature Plants 2019), degradation of lipid droplets (LDs) in germinating seedlings (Plant Cell 2022), and autophagosome closure under stress condition (NC 2022R). We have also identified and characterized several Arabidopsis sof (suppressor of free1) as novel regulators of the MVB pathway (NC 2018; Plant Cell 2019; TBD 2022). In this talk, I will summarize our major findings and present an update on our research progress of multi-functions of FREE1 in Arabidopsis. Supported by Research Grants Council of Hong Kong and CUHK. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Daniël Van Damme If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Vrijdag 3 juni 2022 17:39:52 Onderwerp: You are invited to attend “Multiple functions of the plant unique ESCRT component FREE1 in Arabidopsis thaliana” ON June 23, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Multiple functions of the plant unique ESCRT component FREE1 in Arabidopsis thaliana '' Prof Liwen Jiang Shool of Life Sciences Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong (CUHK) CHINA Thursday, June 23, 2022 14:00 The plant endomembrane system contains several functionally distinct membrane-enclosed organelles, including the endoplasmic reticulum (ER), Golgi apparatus, trans-Golgi network (TGN) or early endosomes (EE), prevacuolar compartment (PVC) or multivesicular body (MVB) and vacuole. The endosomal sorting complex required for transport (ESCRT) machinery mediates the formation of intraluminal vesicles (ILVs) in MVBs as well as the sorting of the ubiquitinated cargoes into the internal vesicles (ILVs) in eukaryotes. Since 2014, we have characterized a plant unique ESCRT component termed FYVE domain protein required for endosomal sorting 1 (FREE1) and demonstrated that FEFF1 plays multiple functions with distinct underlying mechanisms in plants, including regulation of MVB and vacuole biogenesis (Current Biology 2014), crosstalk of the endomembrane system with the autophagic pathway (PNAS 2015), Abscisic Acid (ABA) signaling (Nature Plants 2019), degradation of lipid droplets (LDs) in germinating seedlings (Plant Cell 2022), and autophagosome closure under stress condition (NC 2022R). We have also identified and characterized several Arabidopsis sof (suppressor of free1) as novel regulators of the MVB pathway (NC 2018; Plant Cell 2019; TBD 2022). In this talk, I will summarize our major findings and present an update on our research progress of multi-functions of FREE1 in Arabidopsis. Supported by Research Grants Council of Hong Kong and CUHK. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Daniël Van Damme If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

Kind reminder From: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> To: "meetings" <meetings@psb.vib-ugent.be> Sent: Wednesday, June 22, 2022 2:00:01 PM Subject: Seminar Prof Liwen Jiang TOMORROW at 2 pm in the Schell room REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Vrijdag 3 juni 2022 17:39:52 Onderwerp: You are invited to attend “Multiple functions of the plant unique ESCRT component FREE1 in Arabidopsis thaliana” ON June 23, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Multiple functions of the plant unique ESCRT component FREE1 in Arabidopsis thaliana '' Prof Liwen Jiang Shool of Life Sciences Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong (CUHK) CHINA Thursday, June 23, 2022 14:00 The plant endomembrane system contains several functionally distinct membrane-enclosed organelles, including the endoplasmic reticulum (ER), Golgi apparatus, trans-Golgi network (TGN) or early endosomes (EE), prevacuolar compartment (PVC) or multivesicular body (MVB) and vacuole. The endosomal sorting complex required for transport (ESCRT) machinery mediates the formation of intraluminal vesicles (ILVs) in MVBs as well as the sorting of the ubiquitinated cargoes into the internal vesicles (ILVs) in eukaryotes. Since 2014, we have characterized a plant unique ESCRT component termed FYVE domain protein required for endosomal sorting 1 (FREE1) and demonstrated that FEFF1 plays multiple functions with distinct underlying mechanisms in plants, including regulation of MVB and vacuole biogenesis (Current Biology 2014), crosstalk of the endomembrane system with the autophagic pathway (PNAS 2015), Abscisic Acid (ABA) signaling (Nature Plants 2019), degradation of lipid droplets (LDs) in germinating seedlings (Plant Cell 2022), and autophagosome closure under stress condition (NC 2022R). We have also identified and characterized several Arabidopsis sof (suppressor of free1) as novel regulators of the MVB pathway (NC 2018; Plant Cell 2019; TBD 2022). In this talk, I will summarize our major findings and present an update on our research progress of multi-functions of FREE1 in Arabidopsis. Supported by Research Grants Council of Hong Kong and CUHK. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Daniël Van Damme If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Regulation of tenuazonic acid production in a plant pathogenic fungus, Pyricularia oryzae '' Prof Hiroyuki Osada Department of Pharmaceutical Sciences University of Shizuoka RIKEN Center for Sustainable Resource Science JAPAN Thursday, July 14, 2022 15:00 Rice blast disease is the most important disease of rice worldwide because the disease results in total crop failure. The disease is caused by Pyricularia oryzae ( Magnaporthe oryzae ), a pathogenic fungus. P. oryzae has many secondary metabolism genes but the most secondary metabolites are scarcely produced or not produced under laboratory conditions. Secondary metabolite production might be strictly regulated to produce under specific environmental conditions, such as upon the infection. In my presentation, the induced production of tenuazonic acid, a mycotoxin, in P. oryzae upon the infection will be discussed. 1) Identification of tenuazonic acid biosynthetic gene cluster in P. oryzae . 2) Biosynthetic machinery for tenuazonic acid synthesis. 3) Battle between pathogenic fungus and rice plant. References 1 Yun, C. S., Motoyama, T. & Osada, H. Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme. Nature Commun 6 , 8758 (2015). 2 Yun, C. S., Motoyama, T. & Osada, H. Regulatory Mechanism of Mycotoxin Tenuazonic Acid Production in Pyricularia oryzae. ACS Chem Biol 12 , 2270-2274 (2017). 3 Yun, C. S. et al. Unique features of the ketosynthase domain in a nonribosomal peptide synthetase-polyketide synthase hybrid enzyme, tenuazonic acid synthetase 1. J Biol Chem 295 , 11602-11612 (2020). 4 Kashiwa, T., Motoyama, T., Yoshida, K., Yun, C. S. & Osada, H. Tenuazonic acid production is dispensable for virulence, but its biosynthetic gene expression pattern is associated with the infection of Pyricularia oryzae. Biosci Biotechnol Biochem 86 , 135-139 (2021). 5 Motoyama, T., Yun, C. S. & Osada, H. Biosynthesis and biological function of secondary metabolites of the rice blast fungus Pyricularia oryzae. J Ind Microbiol Biotechnol 48 , kuab058 (2021). Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Cc: "Lieve Ongena" <Lieve.Ongena@vib.be> Verzonden: Donderdag 23 juni 2022 15:29:31 Onderwerp: You are invited to attend “Regulation of tenuazonic acid production in a plant pathogenic fungus, Pyricularia oryzae” ON July 14, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Regulation of tenuazonic acid production in a plant pathogenic fungus, Pyricularia oryzae '' Prof Hiroyuki Osada Department of Pharmaceutical Sciences University of Shizuoka RIKEN Center for Sustainable Resource Science JAPAN Thursday, July 14, 2022 15:00 Rice blast disease is the most important disease of rice worldwide because the disease results in total crop failure. The disease is caused by Pyricularia oryzae ( Magnaporthe oryzae ), a pathogenic fungus. P. oryzae has many secondary metabolism genes but the most secondary metabolites are scarcely produced or not produced under laboratory conditions. Secondary metabolite production might be strictly regulated to produce under specific environmental conditions, such as upon the infection. In my presentation, the induced production of tenuazonic acid, a mycotoxin, in P. oryzae upon the infection will be discussed. 1) Identification of tenuazonic acid biosynthetic gene cluster in P. oryzae . 2) Biosynthetic machinery for tenuazonic acid synthesis. 3) Battle between pathogenic fungus and rice plant. References 1 Yun, C. S., Motoyama, T. & Osada, H. Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme. Nature Commun 6 , 8758 (2015). 2 Yun, C. S., Motoyama, T. & Osada, H. Regulatory Mechanism of Mycotoxin Tenuazonic Acid Production in Pyricularia oryzae. ACS Chem Biol 12 , 2270-2274 (2017). 3 Yun, C. S. et al. Unique features of the ketosynthase domain in a nonribosomal peptide synthetase-polyketide synthase hybrid enzyme, tenuazonic acid synthetase 1. J Biol Chem 295 , 11602-11612 (2020). 4 Kashiwa, T., Motoyama, T., Yoshida, K., Yun, C. S. & Osada, H. Tenuazonic acid production is dispensable for virulence, but its biosynthetic gene expression pattern is associated with the infection of Pyricularia oryzae. Biosci Biotechnol Biochem 86 , 135-139 (2021). 5 Motoyama, T., Yun, C. S. & Osada, H. Biosynthesis and biological function of secondary metabolites of the rice blast fungus Pyricularia oryzae. J Ind Microbiol Biotechnol 48 , kuab058 (2021). Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Dinsdag 31 mei 2022 17:55:00 Onderwerp: You are invited to attend “Challenges and Opportunities of Breeding by Design” ON June 16, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Challenges and Opportunities of Breeding by Design '' Fred Van Ex Vice President R&D Managing Director Inari Agriculture BELGIUM Thursday, June 16, 2022 11:00 Global demand for food and feed is increasing at a rapid pace as a consequence of a growing global population and challenging environmental conditions. Conventional and modern breeding approaches, developed during the Green Revolution, have had a major contribution to the consistent yield increases observed during the past decades but are leveling off and are no longer keeping pace with the growing demands. Reducing genetic diversity and lengthy breeding timelines will further limit what can be achieved through regular breeding. Rapid climate change is putting an additional challenge to what can be achieved in the timelines needed for breeding to obtain high yielding varieties adapted to new environmental conditions and concerns over environmental sustainability will reduce yield gains caused by use of agronomical inputs such as fertilizers and herbicides. Consequently, rapid and significant improvement of varieties need to be achieved through a combination of breeding, improved agronomical practices and new breeding techniques such as gene editing. In this seminar, an overview will be provided of the Inari Agriculture toolbox which is being developed. This toolbox is enabling us to develop improved crop varieties that deliver superior yields with reduced needs of inputs such as water and fertilizer in order to meet the global demands of food security and environmental sustainability. Our toolbox will enable the rapid development of novel allele variation by generating high order multiplex edited populations. Phenotypic selection of plants from this population enables the identification of novel, high order, allele combinations. In addition, the efficient generation of multitype edited plants leads to the generation of novel alleles. This approach already resulted in the identification of interesting genes and alleles linked with improved water and nitrogen use efficiency. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Donderdag 19 mei 2022 18:24:15 Onderwerp: You are invited to attend “Brassinosteroid gene regulatory networks at cellular resolution” ON June 2, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Brassinosteroid gene regulatory networks at cellular resolution '' Dr Trevor Nolan Duke University and Howard Hughes Medical Institute Department of Biology Durham USA Thursday, June 2, 2022 11:00 Brassinosteroids (BRs) are a group of plant steroid hormones that regulate diverse processes such as cell division, cell elongation, and differentiation by controlling the activities of BES1 and BZR1 family transcription factors, which in turn mediate the expression of thousands of genes. The gene regulatory networks (GRNs) that control the diverse processes regulated by BRs are only partially understood, but likely involve additional context-specific regulatory factors. The Arabidopsis root is a tractable model to investigate these networks due to its simple organization and defined cell lineages. We recently constructed an integrated atlas of more than 110,000 cells using single-cell RNA-seq, which highlighted the continuous nature of development in the Arabidopsis root and provided new insights into cell identity acquisition (Shahan, Hsu, et al., 2022, https://doi.org/10.1016/j.devcel.2022.01.008). To further define spatiotemporal BR responses, we performed a time series single-cell RNA-seq experiment following BR treatment. Annotation enabled by our reference atlas allowed us to query BR-regulated gene expression among the majority of cell types and developmental stages of the root. We recovered known hotspots for BR signaling including the epidermis. Our data also indicate that BRs strongly influence gene expression in the cortex, especially in the elongation zone. Waddington-OT reconstruction of cortex trajectories showed that BRs trigger a shift from proliferation to elongation which is associated with increased expression of cell wall-related genes. Accordingly, loss of BR signaling in the cortex has little effect on meristem cell length but impairs cell expansion in the elongation zone. To discover regulators in the elongating cortex, we used CellOracle to infer GRN configurations across each cell type, developmental stage, and time point of our BR time series. Our GRNs and subsequent experimental analysis revealed two transcription factors that play a prominent role in cortex GRNs and affect BR-mediated cell elongation. Finally, we used scRNA-seq to define cell-type-specific changes in gene expression associated with reduced elongation in the cortex of our transcription factor mutants. When combined, these datasets represent more than 200,000 single-cell transcriptomes, providing a view of brassinosteroid-mediated gene expression at unprecedented resolution. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Jenny Russinova If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Donderdag 5 mei 2022 16:37:43 Onderwerp: You are invited to attend “Plasma Membrane to Proline Metabolism: Signaling and metabolic mechanisms that control plant growth and drought resistance” ON May 30, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Plasma Membrane to Proline Metabolism: Signaling and metabolic mechanisms that control plant growth and drought resistance '' Prof Paul E. Verslues Institute of Plant and Microbial Biology Academia Sinica Taipei TAIWAN Monday, May 30, 2022 11:00 In this seminar Prof Paul E. Verslues will present two ongoing projects in our efforts to understand how plants adjust their growth and metabolism in response to moderate severity low water potential (drought) stress. While this type of stress typically does not lead to death of the plant, how the plant responds influences agronomic productivity and ability to adapt to changing environmental conditions. To better understand the role of proline metabolism in drought resistance and its regulation, we performed a forward genetic screen using a reporter driven by the promoter of Proline Dehydrogenase 1 ( ProDH1 pro :LUC; Shinde et al., 2016) and identified a low water potential stress hypersensitive mutant having a single amino acid change in the Non-Phototrophic Hypocotyl3 (NPH3)-domain of an NRL protein. The hypersensitive phenotype could be alleviated by blocking proline metabolism . The nrl point mutation disrupts NRL interaction with RAB small GTPases which are involved in endo-membrane trafficking. These results have implications for both stress biology and plant signaling. For stress biology, our results show that high levels of proline accumulation often seen during drought have the potential to elicit cell death responses unless specific mechanisms are activated to allow proline to accumulate safely. In terms of signaling, our results indicate that the NPH3 domain has previously unknown functions related to membrane trafficking which will enlighten research on the role of other NPH3 proteins. The plasma membrane-localized Clade E Growth-regulating (EGR) type 2C protein phosphatases and their target protein MASP1 have opposing effects on growth during drought stress (Bhaskara et al., 2017). EGRs and MASP1 have opposing gradients of expression across the root meristem and opposing effects on root meristem size at low water potential. Ectopic MASP1 or EGR expression increased or decreased, respectively, root meristem size and root elongation during low water potential stress. However, ectopic EGR expression had no effect on unstressed plants. Phosphomimic MASP1 could overcome EGR suppression of root meristem size. Together these results indicated that during low water potential leads to EGR activation and attenuation of MASP1 phosphorylation to regulate meristem size and activity (Longkumer et al., 2022). References Bhaskara GB, Wen T-N, Nguyen TT, Verslues PE (2017) Protein Phosphatase 2Cs and Microtubule-Associated Stress Protein 1 control microtubule stability, plant growth, and drought response. Plant Cell 29: 169-191 Longkumer T, Chen C-Y, Biancucci M, Bhaskara GB, Verslues PE (2022) Spatial differences in stoichiometry of EGR phosphatase and Microtubule-Associated Stress Protein 1 control root meristem activity during drought stress. Plant Cell : 34: 742–758 Shinde S, Villamor JG, Lin W-D, Sharma S, Verslues PE (2016) Proline coordination with fatty acid synthesis and redox metabolism of chloroplast and mitochondria. Plant Physiology 172: 1074-1088 . Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Hilde Nelissen If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]

REMINDER Van: "Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be> Aan: "meetings" <meetings@psb.vib-ugent.be> Verzonden: Vrijdag 22 april 2022 10:46:08 Onderwerp: You are invited to attend “Avoiding unnecessary stress: post-transcriptional regulation of mitochondrial dysfunction responses in plants” ON May 12, 2022 You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] . '' Avoiding unnecessary stress: post-transcriptional regulation of mitochondrial dysfunction responses in plants '' Associate Prof Saijaliisa Kangasjärvi Faculty of Biological and Environmental Sciences University of Helsinki FINLAND Thursday, May 12, 2022 11:00 Saijaliisa Kangasjärvi 1,2,3 1 Faculty of Biological and Environmental Sciences, Organismal and Evolutionary Biology Research Programme, University of Helsinki, Finland. 2 Faculty of Agriculture and Forestry, Department of Agricultural Sciences, University of Helsinki, Finland. 3 Viikki Plant Science Center, University of Helsinki, Finland Organellar retrograde signals are vital in determining stress reactions in plants. We have identified Protein Phosphatase 2A (PP2A) as a cytosolic factor that controls organelle-induced stress reactions and pathogenesis responses in Arabidopsis thaliana. PP2A regulatory subunit B’γ (PP2A-B’γ) is required to maintain growth and prevent premature senescence under favorable conditions. On a molecular level, PP2A-B’γ controls multiple phosphoproteins that are elicited by mitochondrial dysfunction and critical in determining metabolic activities and detoxification capacity in plant cells. Under stress, the promoter of PP2A-B’γ becomes transiently inactivated by mitochondrial dysfunction signals. Hence, PP2A-B’γ does not prevent stress responses upon environmental challenges, but is an important regulator of stress recovery. Phosphoproteomic analysis suggests networks related to plant immunity, vesicle transport and RNA metabolism as regulation targets for PP2A-B’γ. PP2A-B’γ as a post-transcriptional regulator of mitochondrial dysfunction responses and stress recovery will be presented. Jozef Schell seminar room Technologiepark 71 - 9052 Invited by Prof Dirk Inzé and Prof Frank Van Breusegem If you do not wish to receive this information anymore, please unsubscribe from future mailings at [ https://maillist.psb.ugent.be/mailman/listinfo/seminars | https://maillist.psb.ugent.be/mailman/listinfo/seminars ] Genome editing, cutting-edge technology for a sustainable agriculture VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 9052 Ghent-Belgium Phone: +32(0)9 331 38 00 [ http://www.psb.vib-ugent.be/ | https://www.psb.ugent.be/ ]
participants (3)
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Delphine Verspeel
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Dirk Inze
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VERSPEEL Delphine